| 欧阳洁媛,肖琳琳,刘姿琴,彭果然,薛晓,周春吉,刘鑫.柴朴汤对哮喘小鼠支气管上皮细胞炎症因子及HMGB1/TLR4/NF-κB的影响.[J].中南医学科学杂志.,2024,(3):329-332. |
| 柴朴汤对哮喘小鼠支气管上皮细胞炎症因子及HMGB1/TLR4/NF-κB的影响 |
| The influence of Chaipu decoction on inflammatory factors and HMGB1/TLR4/NF-κ B in bronchial epithelial cells of asthmatic mice |
| 投稿时间：2023-11-28 修订日期：2024-03-28 |
| DOI：10.15972/j.cnki.43-1509/r.2024.03.004 |
| 中文关键词: 柴朴汤 炎症因子 HMGB1 TLR4 NF-κB 小鼠支气管上皮细胞 哮喘 [ |
| 英文关键词:Chaipu decoction inflammation factor HMGB1 TLR4 NF-κB mouse bronchial epithelial cells asthma |
| 基金项目:国家自然科学基金资助项目(81873282)；南华大学临床医学研究“4310”计划(20214310NHYCG04)；国家中医药管理局第七批全国老中医药专家学术经验继承工作项目(国中医药人教函〔2022〕76号) |
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| 中文摘要: |
| 目的探讨柴朴汤对哮喘小鼠支气管上皮细胞高迁移率族蛋白B1(HMGB1)、Toll样受体4(TLR4)、核因子-κB(NF-κB)及下游炎症介质单核细胞趋化蛋白-1(MCP-1)、白细胞介素-6(IL-6)的影响。 方法将小鼠支气管上皮细胞分为对照组(Con组,空白血清培养)、Model组(哮喘小鼠血清培养)和Chaipu组(柴朴汤药血清培养)。采用qRT-PCR和Western blotting检测小鼠支气管上皮细胞中HMGB1、TLR4、NF-κB、MCP-1、IL-6的mRNA及蛋白表达情况,ELISA法测定细胞培养液中MCP-1和IL-6的水平。 结果与Con组相比,Model组小鼠支气管上皮细胞HMGB1、TLR4、NF-κB、MCP-1、IL-6表达均明显升高(P＜0.05)；Model组细胞培养液中MCP-1、IL-6也显著升高(P＜0.05)。与Model组相比,Chaipu组小鼠支气管上皮细胞HMGB1、TLR4、MCP-1、IL-6 mRNA和蛋白表达降低,NF-κB mRNA表达亦降低(P＜0.05)；Chaipu组细胞培养液中MCP-1、IL-6也显著降低(P＜0.05)。 结论柴朴汤可下调哮喘血清诱导的炎症因子的表达,可能与其抑制HMGB1/TLR4/NF-κB炎症通路有关。 |
| 英文摘要: |
| AimTo investigate the effects of Chaipu decoction on high mobility group protein B1 (HMGB1), Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB), and downstream inflammatory mediators monocyte chemotactic protein-1 (MCP-1) and interleukin-6 (IL-6) in bronchial epithelial cells of asthmatic mice. MethodsThe mouse bronchial epithelial cells were divided into a control group (Con group, treated with blank serum culture), a Model group (treated with asthma tic mice serum culture), and a Chaipu group (treated with Chaipu decoction containing serum culture). qRT-PCR and Western blotting were used to detect the mRNA and protein expression of HMGB1, TLR4, NF-κB, MCP-1, and IL-6 in mouse bronchial epithelial cells, and ELISA assay was used to determine the levels of MCP-1 and IL-6 in the cell culture fluid. ResultsCompared with the Con group, the expression of HMGB1, TLR4, NF-κB, MCP-1, and IL-6 in mouse bronchial epithelial cells in the Model group were significantly increased (P＜0.05). The MCP-1 and IL-6 of the cell culture fluid in the Model group were also significantly increased (P＜0.05). The mRNA and protein expression of HMGB1, TLR4, MCP-1, IL-6 were decreased and the expression of NF-κB mRNA was decreased in the Chaipu group compared with the Model group (P＜0.05). The levels of MCP-1 and IL-6 in Chaipu group cell culture medium were also significantly decreased (P＜0.05). ConclusionChaipu decoction can downregulate the expression of inflammatory factors induced by asthma serum, which may be related to its inhibition of HMGB1/TLR4/NF-κ B inflammatory pathway. |
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