| 欧意权,胡泽慧,李章展,龙思鸿,刘禹,陈一鹏,向垚,姚智升,赵维超,龙鼎新.人参皂苷Rg1通过抑制TTR延缓X射线颅脑照射后小鼠海马衰老.[J].中南医学科学杂志.,2024,(3):318-323. |
| 人参皂苷Rg1通过抑制TTR延缓X射线颅脑照射后小鼠海马衰老 |
| Ginsenoside Rg1 delayed hippocampal aging in mice after X-ray cranial irradiation by inhibiting TTR |
| 投稿时间：2024-03-02 修订日期：2024-04-22 |
| DOI：10.15972/j.cnki.43-1509/r.2024.03.002 |
| 中文关键词: 颅脑照射 人参皂苷Rg1 甲状腺素视黄质运载蛋白 海马 衰老 认知功能 [ |
| 英文关键词:cranial irradiation ginsenoside Rg1 TTR hippocampal senescence cognitive function |
| 基金项目:国家自然科学基金(81673227)；湖南省自然科学基金(2020JJ4080和2023JJ30497)；湖南省生态环境厅项目(HBKT-2022021)；湖南省研究生创新基金项目(CX20221026和CX20230994) |
| 作者 | 单位 | E-mail | | 欧意权 | 南华大学衡阳医学院公共卫生学院 典型环境污染与健康危害湖南省重点实验室,湖南衡阳421001 | e-mail为741400910@qq.com,e-mail为59579883@qq.com,e-mail为dxlong99@163.com | | 胡泽慧 | 南华大学衡阳医学院公共卫生学院 典型环境污染与健康危害湖南省重点实验室,湖南衡阳421001 | | | 李章展 | 南华大学衡阳医学院附属南华医院放疗中心,湖南衡阳421002 | | | 龙思鸿 | 南华大学衡阳医学院公共卫生学院 典型环境污染与健康危害湖南省重点实验室,湖南衡阳421001 | | | 刘禹 | 南华大学衡阳医学院公共卫生学院 典型环境污染与健康危害湖南省重点实验室,湖南衡阳421001 | | | 陈一鹏 | 南华大学衡阳医学院公共卫生学院 典型环境污染与健康危害湖南省重点实验室,湖南衡阳421001 | | | 向垚 | 南华大学衡阳医学院公共卫生学院 典型环境污染与健康危害湖南省重点实验室,湖南衡阳421001 | | | 姚智升 | 南华大学衡阳医学院公共卫生学院 典型环境污染与健康危害湖南省重点实验室,湖南衡阳421001 | | | 赵维超 | 南华大学衡阳医学院公共卫生学院 典型环境污染与健康危害湖南省重点实验室,湖南衡阳421001 | e-mail为741400910@qq.com,e-mail为59579883@qq.com,e-mail为dxlong99@163.com | | 龙鼎新 | 南华大学衡阳医学院公共卫生学院 典型环境污染与健康危害湖南省重点实验室,湖南衡阳421001 | e-mail为741400910@qq.com,e-mail为59579883@qq.com,e-mail为dxlong99@163.com |
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| 中文摘要: |
| 目的探讨人参皂苷Rg1延缓X射线颅脑照射后小鼠海马衰老的可能机制。 方法将40只C57BL/6J小鼠随机均分为空白对照组(Con组)、颅脑照射组(IR组)、人参皂苷Rg1预处理组(Rg1组)、人参皂苷Rg1预处理颅脑照射组(Rg1+IR组)。观察各组小鼠体质量、进食情况、活动状态、精神状态、毛色与大小便评估健康状况；采用Morris水迷宫评估小鼠认知能力；采用SA-β-Gal染色检测海马齿状回衰老细胞数量；采用转录组测序分析确定Rg1抗辐射衰老的靶点；采用qRT-PCR和蛋白免疫印迹检测靶点的表达水平。 结果与Con组相比,IR组小鼠体质量减轻、进食量减少、活动减少、精神倦怠、毛发灰暗、大便稀溏,出现明显的认知能力下降,海马齿状回衰老细胞增多(P＜0.05)。与IR组相比,Rg1+IR组小鼠上述指标均有改善(P＜0.05)。甲状腺素视黄质运载蛋白(TTR)为筛选出的最显著靶点。IR组小鼠海马中TTR表达较Con组升高(P＜0.05),而Rg1+IR组TTR表达较IR组降低(P＜0.05)。 结论人参皂苷Rg1可能通过抑制TTR延缓X射线颅脑照射后小鼠的海马衰老,从而保护认知能力。 |
| 英文摘要: |
| AimTo investigate the potential mechanisms by which ginsenoside Rg1 delays X-ray cranial irradiation-induced hippocampal senescence in mice. Methods40 C57BL/6J mice were randomly divided into the control group (Con group), the cranial irradiation group (IR group), the Rg1 pretreatment group (Rg1 group), and the Rg1 pretreatment plus cranial irradiation group (Rg1+IR group). The general health status of the mice, including body weight, food intake, activity, mental state, and fur and excreta, was observed and evaluated. Cognitive function was assessed by using the Morris water maze test, and the number of senescent cells in the hippocampal dentate gyrus was detected by β-galactosidase staining. RNA sequencing (RNA-seq) analysis was used to determine the target of Rg1 against radiation senescence. qRT-PCR and Western blotting were used to detect the expression levels of the targets. ResultsCompared with the Con group, the IR group showed decreased body weight, reduced food intake, decreased activity, mental fatigue, dull fur, and loose stools, as well as significant cognitive impairment and increased hippocampal dentate gyrus senescent cells (P＜0.05). Compared with the IR group, the Rg1+IR group exhibited improvements in all the aforementioned indicators (P＜0.05). Transthyretin (TTR) was identified as the most significant target . The expression of TTR in the hippocampus of IR group mice was increased compared with the control group (P＜0.05), while the Rg1+IR group was decreased compared with the IR group (P＜0.05). ConclusionGinsenoside Rg1 attenuated X-ray-induced hippocampal senescence and protected cognitive function in mice by suppressing the expression of TTR. |
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