| 唐丽,于慧静,王德文.白花蛇舌草诱导活性氧类物质抑制慢性髓系白血病K562细胞增殖.[J].中南医学科学杂志.,2024,(2):193-197. |
| 白花蛇舌草诱导活性氧类物质抑制慢性髓系白血病K562细胞增殖 |
| Mechanism of hedyotis diffusa willd inhibiting the proliferation of K562 cells in chronic myeloid leukemia by inducing reactive oxygen species |
| 投稿时间：2023-05-09 修订日期：2024-01-20 |
| DOI：10.15972/j.cnki.43-1509/r.2024.02.008 |
| 中文关键词: 慢性髓系白血病 K562细胞 白花蛇舌草 细胞增殖 活性氧 [ |
| 英文关键词:chronic myeloid leukemia K562 cell hedyotis diffusa willd cell proliferation reactive oxygen specie |
| 基金项目:威海市文登区科技发展计划项目(20210453) |
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| 中文摘要: |
| 目的探讨白花蛇舌草(HDW)诱导活性氧类物质对慢性髓系白血病(CML)K562细胞增殖的影响。 方法流式细胞法检测HDW作用下K562细胞内活性氧(ROS)水平。将K562细胞分为对照组、HDW组、N-乙酰-L-半胱氨酸组(NAC组)和HDW+NAC组,观察各组细胞ROS、活力、形态、增殖和细胞周期分布情况,Western blotting法检测细胞周期相关蛋白和磷酸化磷脂酰肌醇3-激酶(p-PI3K)、磷酸化丝氨酸苏氨酸激酶(p-Akt)蛋白表达情况。 结果HDW能提高K562细胞内ROS水平(P＜0.05)。对照组、NAC组细胞含大量拒染细胞且形态无明显变化,HDW组、HDW+NAC组拒染细胞减少,着色细胞增多,有染色质聚集和细胞核膜崩解现象。与对照组相比,HDW组细胞内ROS、G1期占比、p21和p53蛋白表达升高,S期占比和细胞周期素D1、细胞周期素依赖蛋白激酶4、p-PI3K、p-Akt蛋白表达降低(P＜0.05)；NAC干预能部分逆转HDW作用效果(P＜0.05)。 结论HDW能诱导活性氧类物质升高来降低K562细胞增殖能力,与细胞周期阻滞、调节PI3K/Akt通路活性有关。 |
| 英文摘要: |
| AimTo explore the effects of hedyotis diffusa willd (HDW) on the proliferation of K562 cells in chronic myeloid leukemia (CML) by inducing reactive oxygen species (ROS). MethodsROS content in K562 cells treated with HDW was detected by flow cytometry. K562 cells were divided into control group, HDW group, N-acetyl-L-cysteine group (NAC group) and HDW+NAC group. ROS content, cells activity, morphology, proliferation and distribution of cells cycle in different groups were measured. The expressions of cells cycle-related proteins, phosphorylated phosphatidylinositol 3-kinase (p-PI3K) and serine threonine kinase (p-Akt) proteins were detected by Western blotting. ResultsHDW could increase ROS content in K562 cells (P＜0.05). In control group and NAC group, there were a large number of trophochrome cells, and there was no significant change in cells morphology. In HDW group and HDW+NAC group, trophochrome cells were reduced, staining cells were increased, and there was chromatin aggregation and nuclear membrane disintegration. Compared with control group, ROS content, proportion of cells in G1 phase and expressions of p21 and p53 proteins were increased, while proportion of cells in S phase and expressions of cyclin D1, cyclin-dependent kinase 4 (CDK4), p-PI3K and P-Akt proteins were decreased in HDW group (P＜0.05). NAC intervention could partially reverse the effect of HDW (P＜0.05). ConclusionHDW can decrease proliferation ability of K562 cells by inducing the increase of ROS, which is related to cell cycle arrest and regulating activity of PI3K/Akt pathways. |
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