| 朱宇凝,屈顺林,曹晓卉,杨志慧,孙桂凤,殷正进,刘仕琦,张缨.IHC、FISH、qRT-PCR检测NSCLC ALK融合基因的对比分析.[J].中南医学科学杂志.,2024,(1):64-67. |
| IHC、FISH、qRT-PCR检测NSCLC ALK融合基因的对比分析 |
| Comparative analysis of IHC, FISH, qRT-PCR for ALK fusion gene detection in NSCLC |
| 投稿时间：2023-05-15 修订日期：2023-12-12 |
| DOI：10.15972/j.cnki.43-1509/r.2024.01.014 |
| 中文关键词: NSCLC ALK融合 IHC FISH qRT-PCR [ |
| 英文关键词:NSCLC ALK fusion IHC FISH qRT-PCR |
| 基金项目:东部战区总医院院内课题(22LCZLXJS17) |
| 作者 | 单位 | E-mail | | 朱宇凝 | 东部战区总医院秦淮医疗区病理科,江苏南京210002 | e-mail为zhuxuena2014@126.com,e-mail为1738876035@qq.com | | 屈顺林 | 南华大学衡阳医学院心血管疾病研究所,湖南衡阳421001 | | | 曹晓卉 | 东部战区总医院秦淮医疗区病理科,江苏南京210002 | | | 杨志慧 | 东部战区总医院秦淮医疗区病理科,江苏南京210002 | | | 孙桂凤 | 东部战区总医院秦淮医疗区病理科,江苏南京210002 | | | 殷正进 | 东部战区总医院秦淮医疗区病理科,江苏南京210002 | | | 刘仕琦 | 东部战区总医院秦淮医疗区病理科,江苏南京210002 | | | 张缨 | 东部战区总医院秦淮医疗区病理科,江苏南京210002 | e-mail为zhuxuena2014@126.com,e-mail为1738876035@qq.com |
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| 中文摘要: |
| 目的对比分析免疫组化(IHC)、荧光原位杂交(FISH)、实时荧光定量PCR(qRT-PCR)检测非小细胞肺癌(NSCLC)ALK融合基因情况。 方法收集453例NSCLC标本。随机选取IHC检测的ALK融合基因不同表达程度标本40例,采用FISH和qRT-PCR法分别检测各组ALK融合基因表达水平,并进行一致性分析。 结果IHC与FISH、qRT-PCR的ALK融合基因检测结果比较,IHC ALK(－)/(3+)与FISH、qRT-PCR检测结果一致率均为100%,具有高度一致性(r=0.781、r=0.740,P＜0.05)。FISH与qRT-PCR的检测结果一致率为97.5%,具有高度一致性(r=0.943,P＜0.05)。 结论FISH、qRT-PCR法与IHC法检测在不同表达程度ALK融合基因样本中检测结果存在差别,IHC敏感性更高。FISH、qRT-PCR法在ALK融合基因样本的检测结果具有高度一致性。 |
| 英文摘要: |
| AimTo comparatively analyze the detection of ALK fusion gene in non-small cell lung cancer (NSCLC) using immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), real-time quantitative polymerase chain reaction (qRT-PCR). MethodsA total of 453 NSCLC specimens were collected. Forty specimens with different levels of ALK fusion gene expression detected by IHC [ALK (－), (+), (2+), (3+), 10 specimens each] were randomly selected. FISH and qRT-PCR were employed to detect the expression levels of ALK fusion genes, and consistency analysis was conducted. ResultsWhen comparing the results of ALK fusion gene detection between IHC and FISH/qRT-PCR, the concordance rate for IHC ALK(－)/(3+) with FISH and qRT-PCR was 100%, demonstrating high consistency (r=0.781, r=0.740, P＜0.05). The concordance rate between FISH and qRT-PCR was 97.5%, showing high consistency (r=0.943, P＜0.05). ConclusionThere are variations in the results of ALK fusion gene detection among samples with different expression levels using FISH, qRT-PCR, and IHC methods, with IHC demonstrating higher sensitivity. FISH and qRT-PCR show high consistency in the detection of ALK fusion gene samples. |
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