| 李媛,苗晓红.JMJD2B、HPIP对宫颈癌Hela细胞生物学行为的影响.[J].中南医学科学杂志.,2024,(1):60-63. |
| JMJD2B、HPIP对宫颈癌Hela细胞生物学行为的影响 |
| Effects of JMJD2B and HPIP on biological behaviors of Hela cells in cervical cancer |
| 投稿时间：2022-11-04 修订日期：2023-11-10 |
| DOI：10.15972/j.cnki.43-1509/r.2024.01.013 |
| 中文关键词: JMJD2B HPIP 宫颈癌 细胞生物学行为 [ |
| 英文关键词:JMJD2B HPIP cervical cancer biological behavior of cell |
| 基金项目:河北省医学科学研究课题计划(20211132) |
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| 中文摘要: |
| 目的分析JMJD2B、HPIP对宫颈癌Hela细胞生物学行为的影响。 方法将Hela细胞分为si-NC组、si-JMJD2B组和si-HPIP组。实时荧光定量PCR检测宫颈癌细胞中JMJD2B、HPIP mRNA表达水平。Western blotting检测宫颈癌细胞和人正常宫颈上皮细胞JMJD2B、HPIP蛋白表达水平。CCK-8检测Hela细胞增殖能力。Transwell检测Hela细胞迁移和侵袭能力。 结果JMJD2B、HPIP蛋白在宫颈癌细胞中表达高于人正常宫颈上皮细胞(P＜0.05)。与si-NC组比较,si-JMJD2B组和si-HPIP组JMJD2B、HPIP mRNA和蛋白表达、细胞增殖、迁移细胞数量、侵袭细胞数量均明显减少(P＜0.05)。 结论JMJD2B和HPIP表达可能调节宫颈癌Hela细胞增殖、迁移和侵袭等生物学行为。 |
| 英文摘要: |
| AimTo analyze the effects of JMJD2B and HPIP on biological behaviors of Hela cells in cervical cancer. MethodsHela cells were divided into si-NC group, si-JMJD2B group and si-HPIP group. The mRNA levels of JMJD2B and HPIP in cervical cancer cells were detected by real-time fluorescence quantitative PCR. The expression levels of JMJD2B and HPIP proteins in cervical cancer cells and normal cervical cells were detected by Western blotting. The proliferation ability of Hela cells was detected by CCK-8. The migration and invasion of Hela cells were detected by Transwell system. ResultsThe expression levels of JMJD2B and HPIP in cervical cancer cells were higher than those in normal cervical cells (P＜0.05). Compared with si-NC group, mRNA and proteins of JMJD2B and HPIP, cells proliferation, number of migration cells and invasion cells were significantly decreased in si-JMJD2B group and si-HPIP group (P＜0.05). ConclusionJMJD2B and HPIP may regulate biological behaviors (including proliferation, migration, invasion) of Hela cells in cervical cancer. |
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