| 张敬,刘殿卿,黄菲,李欣,卢东.呼吸道感染细菌联合疫苗制备及其免疫效果评价.[J].中南医学科学杂志.,2024,(1):40-44. |
| 呼吸道感染细菌联合疫苗制备及其免疫效果评价 |
| Preparation and immune efficacy evaluation of respiratory tract infection bacterial combination vaccine |
| 投稿时间：2023-02-15 修订日期：2023-10-28 |
| DOI：10.15972/j.cnki.43-1509/r.2024.01.009 |
| 中文关键词: 呼吸道感染 细菌联合疫苗 免疫效果 [ |
| 英文关键词:respiratory infections bacterial combination vaccines immune effect |
| 基金项目:河北省卫生健康委医学科学研究课题(20201552) |
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| 中文摘要: |
| 目的探讨呼吸道感染细菌联合疫苗对免疫效果的影响。 方法分离培养6种呼吸道感染细菌(溶血性链球菌、流感嗜血杆菌B型、肺炎链球菌、金黄色葡萄球菌、铜绿假单胞菌及肺炎克雷伯菌),制成联合疫苗。根据联合疫苗质量浓度分为对照组(生理盐水)、低水平组(0.1 g/L疫苗)、中水平组(0.3 g/L疫苗)及高水平组(0.5 g/L疫苗)。各组疫苗作用于人外周血单个核细胞,检测外周血单个核细胞增殖活性、淋巴细胞表型的变化及细胞因子水平。各组疫苗作用于SPF级小鼠,测定各组小鼠迟发型超敏反应、脾脏指数、胸腺指数、巨噬细胞吞噬指数及血清溶血素含量。 结果人外周血各组单个核细胞增殖活性、γ干扰素、白细胞介素-2水平均随时间延长而升高,且在相同时间,联合疫苗质量浓度越高,各指标水平越高(P＜0.05)。与对照组比较,低、中、高水平组单个核细胞CD4＋、CD19＋水平升高,CD8＋水平降低,且随着联合疫苗质量浓度越高,各指标水平变化越明显(P＜0.05)。与对照组小鼠比较,低、中、高水平组小鼠迟发型超敏反应、脾脏指数、胸腺指数、吞噬指数、溶血素水平升高,且随着联合疫苗质量浓度越高,各指标水平越高(P＜0.05)。 结论呼吸道感染细菌联合疫苗有利于提高细胞及体液免疫能力,且联合疫苗质量浓度为0.5 g/L时,可获得的免疫效果最佳。 |
| 英文摘要: |
| AimTo investigate the effect of respiratory infection bacterial combination vaccine on immune efficacy. MethodsSix types of respiratory tract infection bacteria (Streptococcus hemolyticus, Haemophilus influenzae type B, Streptococcus pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae) were isolated and cultured to produce bacterial vaccines. According to the mass concentration of the combined vaccine, they were divided into the control group (physiological saline), low-level group (0.1 g/L vaccine), medium level group (0.3 g/L vaccine) and high-level group (0.5 g/L vaccine). Each group of vaccines was acted on human peripheral blood mononuclear cells in vitro, and the proliferation activity of mononuclear cells, changes in lymphocyte phenotype, and cytokine levels were detected. Each group of vaccines was acted on SPF grade mice in vivo, the delayed type hypersensitivity response, spleen index, thymus index, phagocytic index, and serum hemolysin content of each group of mice were measured. ResultsThe proliferative activity of mononuclear cells, levels of interferon-γ and interleukin-2 in human peripheral blood of all groups were increased with the increase of time, and with higher mass concentration of combined vaccine, the levels of all indicators were higher in the same time (P＜0.05). Compared with the control group, the levels of CD4＋ and CD19＋ of mononuclear cells in the low, medium and high-level groups were increased, while the levels of CD8＋ were decreased, with higher mass concentration of the combined vaccine, the variation of each index level was more obvious (P＜0.05). Compared with the control group, the delayed type hypersensitivity response, spleen index, thymus index, phagocytosis index and hemolysin optical density of mice in low, medium and high-level groups were increased, and the levels of each index were increased with the higher the mass concentration of combined vaccine (P＜0.05). ConclusionThe bacterial combined vaccine for respiratory tract infection can improve the cellular and humoral immunity, and the best immune effect can be obtained when the concentration of the combined vaccine is 0.5 g/L. |
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