| 王雁,李振宇.Chi-CpG NP的制备及其对rNMB0315蛋白的免疫效果.[J].中南医学科学杂志.,2024,(1):31-35. |
| Chi-CpG NP的制备及其对rNMB0315蛋白的免疫效果 |
| Preparation of Chi-CpG NP and its immune effects on the recombinant protein rNMB0315 |
| 投稿时间：2023-08-27 修订日期：2023-12-22 |
| DOI：10.15972/j.cnki.43-1509/r.2024.01.007 |
| 中文关键词: Chi-CpG NP B群脑膜炎奈瑟菌 rNMB0315蛋白 免疫佐剂 [ |
| 英文关键词:Chi-CpG NP Neisseria meningitides serogroup B rNMB0315 mucosal adjuvant |
| 基金项目:湖南省自然科学基金项目(2023JJ60371) |
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| 中文摘要: |
| 目的制备黏膜免疫佐剂Chi-CpG NP,并检测其对重组B群脑膜炎奈瑟菌0315蛋白(rNMB0315)的免疫效果。 方法用壳聚糖纳米颗粒(Chi NP)包裹免疫刺激分子(CpG-ODN)合成Chi-CpG NP,随后检测其理化性质和安全性。将不同黏膜免疫佐剂与rNMB0315充分混合、吸附,滴鼻免疫雌性BALB/c小鼠,检测PBS组、rNMB0315组、rNMB0315+CpG组、rNMB0315+Chi NP组、rNMB0315+Chi-CpG NP组小鼠免疫42天后的体液免疫(IgG、IgG1、IgG2a、sIgA)、细胞免疫水平[白细胞介素(IL)-4、γ干扰素(IFN-γ)、IL-17A]、免疫保护效果以及血清体外杀菌活性。 结果成功制备的Chi-CpG NP呈球形,大小均一,有较好的吸附性能和良好的安全性。小鼠免疫42天后,体液免疫和细胞免疫水平rNMB0315+Chi-NP组、rNMB0315+Chi-CpG NP组高于rNMB0315组(P＜0.05)；rNMB0315+Chi-CpG NP组高于rNMB0315+CpG组和rNMB0315+Chi-NP组(P＜0.05)。致死剂量B群脑膜炎奈瑟菌攻击各组小鼠后,rNMB0315+Chi-CpG NP组小鼠存活率最高；血清体外杀菌抗体滴度为1∶4。 结论成功制备了Chi-CpG NP。Chi-CpG NP能增强rNMB0315蛋白的免疫效果,提高小鼠的存活率。 |
| 英文摘要: |
| AimTo prepare the mucosal adjuvants of Chi-CpG NP, and subsequently immunize mice with recombinant Neisseria meningitides serogroup B 0315 protein (rNMB0315) by using Chi-CpG NP before the observation of immunostimulatory effects. MethodsThe physicochemical characteristics and safety of Chi-CpG NP were assessed by incorporating immunopotentiators (CpG-ODN) into Chitosan nanoparticles (Chi NP). Female BALB/c mice were immunized intranasally with various mucosal immune adjuvants containing the rNMB0315 protein. On days 42 days after immunization, the humoral (IgG, IgG1, IgG2a, sIgA), cellular ( interleukin 4, interferon-γ, interleukin 17A) immunologic responses, as well as the serum bactericidal assay and immune protective effect, were evaluated in the PBS, rNMB0315, rNMB0315+CpG, rNMB0315+Chi NP, and rNMB0315+Chi-CpG NP groups. ResultsThe Chi-CpG nanoparticles demonstrated favorable adsorption and safety properties and exhibited a spherical morphology. On days 42 days after immunization, the levels of humoral and cellular immunity in the rNMB0315+Chi NP group and rNMB0315+Chi-CpG NP group were higher than those in the rNMB0315 group (P＜0.05). Moreover, the rNMB0315+Chi-CpG NP group elicited a higher level of humoral and cellular immunity than the rNMB0315+CpG, or the rNMB0315+Chi-NP group (P＜0.05).After a lethal dose of Neiseria meningitids serogroup B infection, the survival rate of mice in the rNMB0315+Chi-CpG NP group was the highest, with serum bactericidal titers of 1∶4 against this strain. ConclusionChi-CpG NP has been successfully prepared. Chi-CpG NP can enhance the immune effects of rNMB0315 protein and improve the survival rate of mice. |
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