| 张灵,范凌,郝杰,张阳,靳艳丽,高炳华.DLBCL患者血Th22细胞、IL-22水平及IL-22对DLBCL细胞株增殖和侵袭的影响.[J].中南医学科学杂志.,2023,(6):912-915. |
| DLBCL患者血Th22细胞、IL-22水平及IL-22对DLBCL细胞株增殖和侵袭的影响 |
| The levels of Th22 cells and IL-22 in blood of DLBCL patients and the effect of IL-22 on DLBCL cell lines proliferation and invasion |
| 投稿时间：2023-02-27 修订日期：2023-09-25 |
| DOI：10.15972/j.cnki.43-1509/r.2023.06.027 |
| 中文关键词: 弥漫性大B细胞淋巴瘤 Th22细胞 增殖 侵袭 IL-22 [ |
| 英文关键词:diffuse large B-cell lymphoma Th22 cells proliferation invasion IL-22 |
| 基金项目:张家口市科技计划项目(2221074D) |
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| 中文摘要: |
| 目的分析弥漫性大B细胞淋巴瘤(DLBCL)患者血辅助性T细胞22(Th22)细胞、白细胞介素-22(IL-22)水平及IL-22对DLBCL细胞株增殖和侵袭的影响。 方法选择DLBCL患者40例(DLBCL组),按照临床分期分为不同亚组；另选同期体检健康者40例为对照组。流式细胞术检测各组Th22细胞比例,ELISA检测各组IL-22蛋白水平。MTT实验检测DLBCL细胞增殖情况,Transwell实验检测DLBCL细胞侵袭情况。 结果与对照组比较,DLBCL组Th22细胞比例和IL-22水平升高；与Ⅰ~Ⅱ期比较,Ⅲ~Ⅳ期Th22细胞比例和IL-22水平升高(P＜0.05)。化疗后Th22细胞比例低于化疗前,复发者Th22细胞比例高于新诊患者和对照组(P＜0.05)。MTT和Transwell实验结果显示,IL-22促进DLBCL细胞的增殖和侵袭(P＜0.05)。 结论DLBCL患者Th22细胞比例及其细胞因子IL-22水平升高,IL-22促进DLBCL细胞增殖和侵袭,为DLBCL研究提供新策略。 |
| 英文摘要: |
| AimTo analyze the levels of helper T cell 22 (Th22) cells and interleukin-22 (IL-22) in patients with diffuse large B-cell lymphoma (DLBCL), and the effects of IL-22 on the proliferation and invasion of DLBCL cell lines. MethodsForty patients with DLBCL (DLBCL group) were selected and divided into different subgroups according to clinical staging, and another 40 healthy individuals who underwent physical examination during the same period were selected as the control group. Flow cytometry was used to detect the level of Th22 cells in each group, and ELISA was used to detect the level of IL-22 protein in each group. MTT assay was used to detect the proliferation of DLBCL cells, and Transwell assay was used to detect the invasion of DLBCL cells. ResultsCompared with the control group, the levels of Th22 cells and IL-22 in the DLBCL group were increased. Compared with stages Ⅰ－Ⅱ, the levels of T22 cells and IL-22 in stages Ⅲ-Ⅳ were increased (P＜0.05). The proportion of Th22 after chemotherapy was lower than that before, while the proportion of Th22 in relapsed patients was higher than that in newly diagnosed patients and the control group (P＜0.05).The results of MTT and Transwell experiments showed that IL-22 promoted the proliferation and invasion of DLBCL cells (P＜0.05). ConclusionThe levels of Th22 cells and their cytokine IL-22 in DLBCL patients are elevated, and IL-22 promotes the proliferation and invasion of DLBCL cells, providing a new strategy for DLBCL research. |
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