| 毛星荐,高其亮,夏冬静,师权.hsa_circ_100395对胃癌细胞HGC-27、MGC-803增殖、侵袭和迁移的影响.[J].中南医学科学杂志.,2023,(6):858-862. |
| hsa_circ_100395对胃癌细胞HGC-27、MGC-803增殖、侵袭和迁移的影响 |
| Effects of hsa_circ_100395 on proliferation, invasion and migration of HGC-27 and MGC-803 gastric cancer cells |
| 投稿时间：2022-03-15 修订日期：2023-07-12 |
| DOI：10.15972/j.cnki.43-1509/r.2023.06.013 |
| 中文关键词: 胃癌 hsa_circ_100395 增殖 侵袭 迁移 [ |
| 英文关键词:gastric cancer hsa_circ_100395 proliferation invasion migration |
| 基金项目:四川省科技计划项目(2019YFS0082) |
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| 中文摘要: |
| 目的探究hsa_circ_100395对胃癌细胞HGC-27、MGC-803增殖、侵袭和迁移的影响。 方法收集72例胃癌患者胃癌组织及其癌旁组织样本,采用qRT-PCR检测hsa_circ_100395在胃癌组织中的表达情况,并分析其与临床病理特征的关系。将胃癌细胞系HGC-27和MGC-803分成过表达组(转染hsa_circ_100395过表达质粒)、沉默组(转染hsa_circ_100395沉默质粒)和对照组(转染空质粒)。采用CCK-8法、划痕愈合实验、Transwell法、裸鼠成瘤实验检测胃癌细胞增殖、迁移和侵袭能力。 结果hsa_circ_100395在胃癌组织中表达明显下调。hsa_circ_100395低表达与高表达在组织分化程度、浸润深度及TNM分期上差异有显著性(P＜0.05)。与对照组比较,hsa_circ_100395过表达显著抑制HGC-27和MGC-803细胞增殖、迁移、侵袭及体内肿瘤的生长；hsa_circ_100395沉默显著促进了HGC-27和MGC-803细胞增殖、迁移、侵袭及体内肿瘤的生长。 结论hsa_circ_100395在胃癌组织中低表达,且过表达hsa_circ_100395可抑制胃癌细胞HGC-27、MGC-803增殖、侵袭和迁移。 |
| 英文摘要: |
| AimTo explore the effects of hsa_circ_100395 on proliferation, invasion and migration of gastric cancer cells. MethodsThe gastric cancer tissues and adjacent tissue samples were collected from 72 patients with gastric cancer, qRT-PCR was used to detect the expression of hsa_circ_100395 in gastric cancer tissues, and the relationship with clinicopathological characteristics was analyzed. The gastric cancer cell lines HGC-27 and MGC-803 were divided into overexpression group (transfected with hsa_circ_100395 overexpression plasmid), silence group (transfected with hsa_circ_100395 silent plasmid) and control group (transfected with empty plasmid). CCK-8 method, scratch healing test, Transwell method, and nude mouse tumor formation test were used to detect the proliferation, migration and invasion ability of gastric cancer cells. ResultsThe expression of hsa_circ_100395 was significantly down-regulated in gastric cancer. There were significant differences in tissue differentiation, infiltration depth, and TNM staging between low expression and high expression of hsa_circ_100395(P＜0.05). Compared with the control group, the overexpression of hsa_circ_100395 significantly inhibited the proliferation, migration, invasion of HGC-27 and MGC-803 cells and tumor growth, whereas hsa_circ_100395 silence significantly promoted the proliferation, migration, invasion of HGC-27 and MGC-803 cells and tumor growth. ConclusionHsa_circ_100395 is expressed at low level in gastric cancer tissues, and overexpression of hsa_circ_100395 can inhibit the proliferation, invasion and migration of gastric cancer cells. |
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