| 庄亚萍,林志星,钟昌会,邓小彦.花姜酮通过抑制NLRP3炎症小体改善SAP大鼠肠道黏膜屏障功能.[J].中南医学科学杂志.,2023,(6):853-857. |
| 花姜酮通过抑制NLRP3炎症小体改善SAP大鼠肠道黏膜屏障功能 |
| Zingerone ameliorates intestinal mucosal barrier function in SAP rats by inhibiting NLRP3 inflammasome |
| 投稿时间：2022-03-10 修订日期：2023-09-16 |
| DOI：10.15972/j.cnki.43-1509/r.2023.06.012 |
| 中文关键词: 花姜酮 大鼠 重症急性胰腺炎 肠道黏膜屏障 NLRP3炎症小体 [ |
| 英文关键词:zingerone rat severe acute pancreatitis intestinal mucosal barrier NLRP3 inflammasome |
| 基金项目:海南省卫生健康行业科研项目(21A200054) |
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| 中文摘要: |
| 目的探讨花姜酮对重症急性胰腺炎(SAP)大鼠肠道黏膜屏障功能的影响。 方法将42只SD大鼠随机均分为对照组(蒸馏水处理SD大鼠)、模型组(蒸馏水处理SAP大鼠)、花姜酮组(花姜酮处理SAP大鼠)。采用HE染色观察各组大鼠小肠组织病理学变化；ELISA法检测血清和小肠组织中白细胞介素(IL)-1β和肿瘤坏死因子-α(TNF-α)含量；Griess法测定血清一氧化氮(NO)水平；偶氮基质显色法测定内毒素(ET)含量；胶乳增强免疫比浊法测定髓过氧化物酶(MPO)含量；EB染色检测肠道血管通透性；免疫荧光法检测小肠组织和肠系膜淋巴结组织中核苷酸结合寡聚化结构域样受体家族热蛋白结构域蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)阳性细胞数；免疫组织化学染色法检测小肠组织和肠系膜淋巴结组织中Casepase-1蛋白表达水平,以及小肠黏膜组织中分泌型免疫球蛋白A(sIgA)水平。 结果花姜酮组大鼠小肠组织有少量炎症细胞浸润,但组织充血、水肿程度较模型组减轻。与模型组比较,花姜酮组大鼠ET、IL-1β、TNF-α、NO、MPO水平及肠道血管通透性降低(P＜0.05)；小肠组织和肠系膜淋巴结NLRP3、ASC阳性细胞数及Casepase-1蛋白表达水平减少(P＜0.05)；小肠黏膜sIgA蛋白表达水平增加(P＜0.05)。 结论花姜酮对SAP大鼠肠黏膜屏障具有保护作用,其机制可能与上调sIgA水平,抑制NLRP3炎症小体活化,调节肠道免疫功能有关。 |
| 英文摘要: |
| AimTo explore the effect of zingerone on the intestinal mucosal barrier function of rats with severe acute pancreatitis (SAP). Methods42 SD rats were randomly divided into the control group (SD rats treated with distilled water), the model group (SAP rats treated with distilled water), and the zingerone group (SAP rats treated with zingerone). The histopathological changes of the small intestine were detected by HE staining; Interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in serum and small intestine tissue were detected by ELISA. Nitric oxide (NO) level was measured by Griess method. Endotoxemia (ET) content was measured by azo matrix color method. Latex enhancement immunoturbidimetric method was used to determine the content of myeloperoxidase (MPO). EB staining was used to determine intestinal vascular permeability. Immunofluorescence method was used to detect the number of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) and apoptosis-associated speck-like protein containing CARD (ASC) positive cells in small intestine tissue and mesenteric lymph node tissue. Immunohistochemical staining method was used to detect Casepase-1 protein in small intestine tissue and mesenteric lymph node tissue, as well as secretory immunoglobulin A (sIgA) in small intestine mucosal tissue. ResultsThere was a small amount of inflammatory cell infiltration in the small intestine tissue of the zingerone group rats, but the degree of tissue congestion and edema was reduced compared to the model group. Compared with the model group, the levels of ET, IL-1β,TNF- α, NO, MPO and intestinal vascular permeability in the zingerone group were decreased (P＜0.05). The number of NLRP3 and ASC positive cells in small intestine tissue and mesenteric lymph nodes, as well as the expression level of Casepase-1 protein in the zingerone group, were decreased (P＜0.05). The expression level of sIgA protein in the small intestine mucosa in the zingerone group was increased (P＜0.05). ConclusionZingerone has a protective effect on the intestinal mucosal barrier of SAP rats, and zingerone has a protective effect on the intestinal mucosal barrier in SAP rats, which may be related to upregulated sIgA levels, inhibited NLRP3 inflammatory complex activation, and modulated intestinal immune function. |
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