| 李双,谭翠钰,侯连杰,赵国军.褪黑素抑制人血管平滑肌细胞的巨噬样表型转化.[J].中南医学科学杂志.,2023,(6):808-812. |
| 褪黑素抑制人血管平滑肌细胞的巨噬样表型转化 |
| Melatonin inhibits the macrophage-like phenotype transformation of human vascular smooth muscle cells |
| 投稿时间：2023-07-17 修订日期：2023-10-25 |
| DOI：10.15972/j.cnki.43-1509/r.2023.06.003 |
| 中文关键词: 动脉粥样硬化 血管平滑肌细胞 巨噬样表型转化 褪黑素 [ |
| 英文关键词:As vascular smooth muscle cells macrophage-like phenotype transformation melatonin |
| 基金项目:国家自然科学基金面上项目(81870337,82300517)；广州医科大学2022年度学生创新能力提升计划项目(2022-66) |
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| 中文摘要: |
| 目的探究褪黑素在人血管平滑肌细胞(VSMC)巨噬样表型转化中的作用。 方法VSMC分为对照组、胆固醇组(水溶性胆固醇)、褪黑素组(水溶性胆固醇+褪黑素)。水溶性胆固醇诱导VSMC建立巨噬样表型转化模型。定量PCR检测VSMC巨噬样表型相关基因表达水平；油红O染色观察各组VSMC脂质吞噬能力；Edu染色观察各组VSMC增殖情况；细胞划痕实验观察VSMC迁移情况。 结果10 mg/L水溶性胆固醇显著降低平滑肌收缩表型相关标志基因α-actin和α-tropomyosin mRNA表达,并显著升高巨噬细胞相关标志基因CD68和MAC2 mRNA表达,诱导VSMC巨噬样表型转化。0.5 μmol/L褪黑素可抑制水溶性胆固醇引起的VSMC脂质蓄积和细胞增殖,但对VSMC迁移无影响。 结论褪黑素抑制VSMC的巨噬样表型转化。 |
| 英文摘要: |
| AimTo establish a macrophage-like phenotypic transformation model of human vascular smooth muscle cells (VSMC) induced by water-soluble cholesterol, and to explore the role of melatonin in this process. MethodsVSMC is divided into a control group, a cholesterol group (water-soluble cholesterol), and a melatonin group (water-soluble cholesterol+melatonin). Establishment of a macrophage like phenotype transformation model in VSMC induced by water-soluble cholesterol. The expression of macrophage-like phenotypic related genes in VSMC was detected by quantitative PCR. Oil red O staining experiment was conducted to observe the effect of melatonin on the lipid accumulation of VSMC induced by water-soluble cholesterol. Edu staining was used to observe the effect of melatonin on the proliferation of VSMC induced by water-soluble cholesterol. The effect of melatonin on VSMC migration induced by water soluble cholesterol was evaluated by cell scratch assay. ResultsThe mRNA expression of smooth musclecontraction phenotype related markers, α-actin and α-tropomyosin,were significantly decreased after 10 mg/L water-soluble cholesterol treatment, while the mRNA expression macrophage related markers,CD68 and MAC2,were significantly increased after treated by 10 mg/L water-soluble cholesterol, suggesting the transformation of VSMC to a macrophage-like phenotype. In addition, 0.5 μmol/L melatonin inhibited lipid accumulation and proliferation of VSMC induced by water-soluble cholesterol, but had no effect on VSMC migration. ConclusionMelatonin inhibits the macrophage-like phenotype transformation of VSMC. |
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