| 彭依琳,陈文欣,胡华,周建斌.miR-27a低表达对子宫内膜癌HEC-1-A细胞增殖的影响.[J].中南医学科学杂志.,2023,(3):360-362. |
| miR-27a低表达对子宫内膜癌HEC-1-A细胞增殖的影响 |
| The effect of low expression of miR-27a on the proliferation on endometrial cancer HEC-1-A cells |
| 投稿时间：2022-12-09 修订日期：2023-03-29 |
| DOI：10.15972/j.cnki.43-1509/r.2023.03.010 |
| 中文关键词: miR-27a 子宫内膜癌 增殖 HEC-1-A细胞 [ |
| 英文关键词:miR-27a endometrial carcinoma proliferation HEC-1-A cell |
| 基金项目:湖南省自然科学基金项目(2021JJ70112) |
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| 中文摘要: |
| 目的研究miR-27a低表达对子宫内膜癌HEC-1-A细胞增殖的影响。 方法构建miR-27a低表达HEC-1-A细胞株。细胞实验分为空白对照组(HEC-1-A不做任何处理)、NC-miR组(转染miR-27a空载体)及miR-27a inhibitor组(转染miR-27a抑制剂)。qRT-PCR法检测各组细胞miR-27a表达情况；MTT比色法和平板克隆技术检测各组细胞增殖能力。 结果成功构建miR-27a低表达HEC-1-A细胞株。miR-27a inhibitor组miR-27a水平及细胞增殖能力均低于NC-miR组及空白对照组(P＜0.05)。 结论miR-27a低表达抑制子宫内膜癌HEC-1-A细胞增殖能力。 |
| 英文摘要: |
| AimTo study the effect of low miR-27a expression on the proliferation ability of HEC-1-A cells in human endometrial cancer. MethodsLow expression of miR-27a HEC-1-A cell line was established. The experiment was set as blank control group (HEC-1-A without any treatment), NC-miR group (transfected empty vector) and miR-27a inhibitor group (transfected miR-27a inhibitor). The expression of miR-27a in each group of cells was detected by qRT-PCR. The cell proliferation capacity was detected by MTT colorimetric method and plate clone formation method. ResultsmiR-27a low-expression HEC-1-A cell line was successfully constructed. The expression of miR-27a and the cell proliferation capacity in the miR-27a inhibitor group were significantly lower than those in NC-miR group and blank control group (P＜0.05). ConclusionThe low expression of miR-27a inhibits the proliferation of endometrial cancer HEC-1-A cells. |
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