| 张学武,郑茜玲,刘继华.下调miR-409-5p对口腔癌细胞SCC-9增殖、迁移的影响.[J].中南医学科学杂志.,2023,(3):340-343. |
| 下调miR-409-5p对口腔癌细胞SCC-9增殖、迁移的影响 |
| Effect of down-regulating miR-409-5p on proliferation and migration of oral cancer cell line SCC-9 and its mechanism |
| 投稿时间：2022-09-14 修订日期：2023-04-17 |
| DOI：10.15972/j.cnki.43-1509/r.2023.03.006 |
| 中文关键词: 口腔癌 miR-409-5p 增殖 侵袭 迁移 vimentin [ |
| 英文关键词:oral cancer miR-409-5p proliferation invasion and migration vimentin |
| 基金项目:陕西省教育厅专项科研计划项目(16JK1852) |
|
| 摘要点击次数: 182 |
| 全文下载次数: 89 |
| 中文摘要: |
| 目的探讨下调微小核糖核酸-409-5p(miR-409-5p)对人口腔癌细胞SCC-9增殖、迁移的影响。 方法收集口腔癌患者口腔癌组织及癌旁正常组织,采用qRT-PCR检测癌组织及癌旁正常组织中miR-409-5p mRNA相对表达量。qRT-PCR筛选出细胞株SCC-9细胞后,将其分为空白对照组(Lip2000处理SCC-9细胞)、阴性对照组(Lip2000＋阴性对照序列处理SCC-9细胞)、miR-409-5p组(Lip2000和inhibitor-miR-409-5p共同处理SCC-9细胞)。采用MTT法、Transwell小室法检测miR-409-5p对细胞增殖、侵袭、迁移的影响。qRT-PCR法、Western blotting法检测细胞vimentin mRNA和蛋白的相对表达量。 结果与癌旁正常组织比较,口腔癌组织中miR-409-5p显著增加(P＜0.05)。与空白对照组和阴性对照组比较,miR-409-5p组细胞增殖、侵袭和迁移能力均下降(P＜0.05)；miR-409-5p组vimentin mRNA和蛋白相对表达量也显著降低(P＜0.05)。 结论下调miR-409-5p能够抑制SCC-9细胞的增殖、迁移,其机制可能与下调vimentin蛋白表达有关。 |
| 英文摘要: |
| AimTo investigate the effect of down-regulating microRNA-409-5p (miR-409-5p) on the proliferation and migration of human oral cancer cell SCC-9. MethodsOral cancer tissues and adjacent normal tissues from patients with oral cancer were collected, and qRT-PCR was used to detect the relative expression of miR-409-5p mRNA in cancer tissues and adjacent normal tissues. After screening the confirmed cell line SCC-9 cells by qRT-PCR, they were divided into blank control group (Lip2000-treated SCC-9 cells), negative control group (Lip2000+nc sequence-treated SCC-9 cells), miR-409-5p group (SCC-9 cells co-treated with Lip2000 and inhibitor-miR-409-5p). The effects of miR-409-5p on cell proliferation, invasion and migration were detected by MTT assay and Transwell chamber assay. The relative expression of vimentin mRNA and protein was detected by qRT-PCR and Western blotting. ResultsCompared with adjacent normal tissues, miR-409-5p was significantly increased in oral cancer tissues (P＜0.05). Compared with the blank control group and the negative control group, the proliferation, invasion and migration abilities of cells in the miR-409-5p group were decreased (P＜0.05); the relative expressions of vimentin mRNA and protein in the miR-409-5p group were also significantly decreased (P＜0.05). ConclusionDown-regulation of miR-409-5p may inhibit SCC-9 cell proliferation and migration, and the mechanism may be related to down-regulation of vimentin protein-related expression. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |