| 史可靓,方春霞,赵兰华,周辉,李忠玉.SiO2-Pickering乳液佐剂增强沙眼衣原体Pgp3蛋白免疫效果.[J].中南医学科学杂志.,2023,(3):319-324. |
| SiO2-Pickering乳液佐剂增强沙眼衣原体Pgp3蛋白免疫效果 |
| Effect of SiO2-Pickering emulsion adjuvant on enhancing Pgp3 protein immunity of Chlamydia trachomatis |
| 投稿时间：2022-11-25 修订日期：2023-03-25 |
| DOI：10.15972/j.cnki.43-1509/r.2023.03.002 |
| 中文关键词: SPE乳液佐剂 沙眼衣原体 Pgp3蛋白 免疫反应 [ |
| 英文关键词:SPE emulsion adjuvant Chlamydia trachomatis Pgp3 protein immune response |
| 基金项目:国家自然科学基金(82272383、32070189)；湖南省自然科学基金(2021JJ30594)；湖南省教育厅项目(20A421、20C1390)；湖南省卫健委项目(202111000475) |
| 作者 | 单位 | E-mail | | 史可靓 | 南华大学衡阳医学院病原生物学研究所 特殊病原体防控湖南省重点实验室,湖南衡阳 421001 | e-mail为845680273@qq.com,e-mail为281115318@qq.com,e-mail为lzhy1023@hotmail.com | | 方春霞 | 南华大学衡阳医学院病原生物学研究所 特殊病原体防控湖南省重点实验室,湖南衡阳 421001 | | | 赵兰华 | 南华大学衡阳医学院病原生物学研究所 特殊病原体防控湖南省重点实验室,湖南衡阳 421001 | | | 周辉 | 湖南中医药大学第一附属医院,湖南长沙 410007 | e-mail为845680273@qq.com,e-mail为281115318@qq.com,e-mail为lzhy1023@hotmail.com | | 李忠玉 | 南华大学衡阳医学院病原生物学研究所 特殊病原体防控湖南省重点实验室,湖南衡阳 421001 | e-mail为845680273@qq.com,e-mail为281115318@qq.com,e-mail为lzhy1023@hotmail.com |
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| 中文摘要: |
| 目的优化二氧化硅(SiO2)Pickering(SPE)乳液佐剂,研究其对沙眼衣原体(Ct)Pgp3蛋白的免疫增强效应,为预防Ct感染性疾病提供实验依据。 方法优化SiO2质量浓度、水油比及超声功率,超声乳化制备SPE佐剂。30只小鼠均分为SPE+Pgp3组(Pgp3蛋白和SPE混合免疫)、Pgp3组(Pgp3蛋白单独免疫)和PBS组。ELISA检测各组小鼠血清抗体水平和脾细胞上清细胞因子水平,流式细胞术检测脾细胞γ干扰素(IFN-γ)、白细胞介素-4(IL-4)水平。 结果SPE最优制备条件为超声功率337 W、水油比10∶2、SiO2质量浓度1 g/L,最优条件下SPE直径为(300.12±44.26) nm,聚合物分散性指数为0.33±0.12。除PBS组外,其他组均检测到了特异性抗体,SPE+Pgp3组总抗体IgG及其亚类IgG1、IgG2a抗体水平高于Pgp3组,且以IgG2a升高为主(P＜0.05)。SPE+Pgp3组、Pgp3组脾细胞IFN-γ和刺激指数高于PBS组,且SPE+Pgp3组高于Pgp3组(P＜0.05)；IFN-γ主要由CD4＋T细胞和CD8＋T细胞分泌。各组IL-4含量均较低。 结论SPE乳液佐剂具有良好的佐剂效应,能有效增强Pgp3蛋白体液免疫和细胞免疫,且可优势诱导Th1型免疫反应。 |
| 英文摘要: |
| AimTo optimize the emulsion adjuvant of silica (SiO2) Pickering (SPE) and study its immune-enhancing effect on Pgp3 protein of Chlamydia trachomatis (Ct), providing experimental basis for the prevention of Ct infectious diseases. MethodsSPE adjuvant was prepared by phacoemulsification with SiO2 concentration, water-oil ratio and ultrasonic power optimized. All 30 mice were divided into SPE+Pgp3 group(Pgp3 protein and SPE mixed immunization), Pgp3 group (Pgp3 protein alone immunization) and PBS group. Serum antibody levels and splenocyte supernatant cytokine levels were detected by ELISA, and spleen cells interferon-γ (IFN-γ) and interleukin-4 (IL-4) levels were detected by flow cytometry. ResultsThe optimal preparation conditions of SPE were ultrasonic power 337 W, water-oil ratio 10∶2, SiO2 concentration 1 g/L, SPE size diameter (300.12±44.26) nm, polymer dispersion index 0.33±0.12. Specific antibodies were detected in all groups except PBS group. The levels of total IgG and its subclasses IgG1 and IgG2a in SPE+Pgp3 group were higher than those in Pgp3 group, and IgG2a was mainly increased (P＜0.05). The IFN-γ and stimulation indices of spleen cells in SPE+Pgp3 and Pgp3 groups were higher than those in PBS group, and SPE+Pgp3 group was higher than those in Pgp3 group (P＜0.05); IFN-γ is mainly secreted by CD4＋T cells and CD8＋T cells. The content of IL-4 in each group was low. ConclusionSPE emulsion adjuvant had a good adjuvant effect,which effectively enhanced the humoral and cellular immunity induced by Pgp3 protein in mice, and the immune response induced by SPE+Pgp3 vaccine preparation tended to be Th1 type. |
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