| 杨文秀,陈向穗,宫凤英,林捷.楮实子对肝星状细胞增殖、凋亡及TLR4/NF-κB/TNF-α信号通路的影响.[J].中南医学科学杂志.,2023,(2):193-196. |
| 楮实子对肝星状细胞增殖、凋亡及TLR4/NF-κB/TNF-α信号通路的影响 |
| Effects of fructus broussonetiae on the proliferation, apoptosis and TLR4/NF-κB/TNF-α signal pathway of hepatic stellate cells |
| 投稿时间：2021-08-26 修订日期：2022-08-27 |
| DOI：10.15972/j.cnki.43-1509/r.2023.02.009 |
| 中文关键词: 楮实子 肝星状细胞 增殖 凋亡 Toll样受体4/核因子-κB/肿瘤坏死因子-α信号通路 [ |
| 英文关键词:fructus broussonetiae HSC proliferation apoptosis TLR4/NK-κB/TNF-α |
| 基金项目:海南省自然科学基金面上项目(820MS162) |
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| 中文摘要: |
| 目的探究楮实子对肝星状细胞(HSC)增殖、凋亡及Toll样受体4(TLR4)/核因子-κB(NF-κB)/肿瘤坏死因子-α(TNF-α)信号通路的影响。 方法体外培养大鼠肝星状细胞系(HSC-T6),正常培养细胞作为空白组；使用转化生长因子-β1(TGF-β1)刺激细胞24 h后,分别添加不同质量浓度(0、1、5、10 g/L)的楮实子培养细胞,依次作为对照组、低剂量组、中剂量组、高剂量组。MTT法、Annexin V-FITC/PI双染法检测楮实子对HSC-T6细胞增殖、凋亡的影响；ELISA法检测HSC-T6细胞上清液中TNF-α、白细胞介素-6(IL-6)含量；Western blotting法检测HSC-T6细胞中TLR4、NF-κB、TNF-α蛋白表达。 结果楮实子可抑制活化的HSC-T6细胞增殖,促进其凋亡,且呈质量浓度依赖性(P＜0.05)；楮实子可降低活化的HSC-T6细胞上清液中TNF-α、IL-6水平,下调细胞中TLR4、NF-κB、TNF-α蛋白水平,并均呈质量浓度依赖性(P＜0.05)。 结论楮实子可抑制HSC-T6细胞增殖,促进HSC-T6细胞凋亡,其机制可能与抑制TLR4/NF-κB/TNF-α信号通路激活有关。 |
| 英文摘要: |
| AimTo investigate the effects of fructus broussonetiae on proliferation and apoptosis of hepatic stellate cells (HSC) and toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB)/tumor necrosis factor-α (TNF-α) signal pathway. MethodsRat hepatic stellate cell line HSC-T6 was cultured in vitro, and the normal cultured cells were used as the blank group; after stimulating the cells with transforming growth factor-β1 (TGF-β1) for 24 h, different concentrations (0,1, 5, and 10 g/L) of fructus broussonetiae were added to cultured cells to serve as the control group, low concentration group, middle concentration group, and high concentration group. MTT assay and Annexin V-FITC/PI double staining method were used to detect the effects of fructus broussonetiae on the proliferation and apoptosis of HSC-T6 cells; ELISA method was used to detect the contents of TNF-α and Interleukin-6 (IL-6) in the supernatant of HSC-T6 cells; Western blotting method was used to detect the protein expression of TLR4, NF-κB and TNF-α in HSC-T6 cells. ResultsFructus broussonetiae could inhibit the proliferation of activated HSC-T6 cells and promote their apoptosis in a concentration-dependent decline or increase manner (P＜0.05); Fructus broussonetiae could reduce the levels of TNF-α and IL-6 in the supernatant of activated HSC-T6 cells, and down-regulate the levels of TLR4, NF-κB, and TNF-α proteins in the cells, all in a concentration-dependent decline manner (P＜0.05). ConclusionFructus broussonetiae could inhibit the proliferation of HSC-T6 cells and promote the apoptosis of HSC-T6 cells. The mechanism may be related to inhibiting the activation of the TLR4/NF-κB/TNF-α signaling pathway. |
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