| 李祎楠,刘欣艳.二甲双胍通过调节AMPK/ERK通路改善动脉粥样硬化小鼠血管平滑肌细胞异常增殖和动脉粥样硬化.[J].中南医学科学杂志.,2023,(1):32-36. |
| 二甲双胍通过调节AMPK/ERK通路改善动脉粥样硬化小鼠血管平滑肌细胞异常增殖和动脉粥样硬化 |
| Metformin improves the abnormal proliferation of vascular smooth muscle cells and atherosclerosis in atherosclerotic mice by regulating AMPK/ERK pathway |
| 投稿时间：2022-05-20 修订日期：2022-11-29 |
| DOI：10.15972/j.cnki.43-1509/r.2023.01.008 |
| 中文关键词: 二甲双胍 动脉粥样硬化 AMPK/ERK通路 血管平滑肌细胞 增殖 迁移 [ |
| 英文关键词:metformin atherosclerosis AMPK/ERK pathway vascular smooth muscle cells proliferation migration |
| 基金项目:上海市普陀区中心医院科研项目(2020317B) |
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| 中文摘要: |
| 目的探讨二甲双胍对动脉粥样硬化小鼠血管平滑肌细胞异常增殖和动脉粥样硬化的影响和机制。 方法将C57/B6小鼠分为对照组、模型组、二甲双胍组。油红O染色、免疫荧光染色法检测各组小鼠主动脉动脉粥样斑块沉积面积比例以及主动脉组织中Ki67和α-SMA表达情况。CCK-8、划痕实验和Western blotting分析小鼠血管平滑肌细胞增殖、迁移能力以及单磷酸腺苷依赖的蛋白激酶(AMPK)、细胞外调节蛋白激酶(ERK)通路和细胞骨架蛋白Pdlim5的变化。 结果与模型组比较,二甲双胍组小鼠主动脉动脉粥样斑块沉积面积比例、Ki67和α-SMA阳性细胞数、血管平滑肌细胞增殖和迁移能力、ERK水平均降低,而AMPK、Pdlim5水平升高(P＜0.05)。 结论二甲双胍通过调节AMPK和ERK通路改善高脂饮食介导小鼠血管平滑肌细胞异常增殖和动脉粥样硬化。 |
| 英文摘要: |
| AimTo investigate the effect and mechanism of metformin on the abnormal proliferation of vascular smooth muscle cells and atherosclerosis in atherosclerotic mice. MethodsC57/B6 mice were divided into control group, model group and metformin group. Oil red O staining and immunofluorescence staining were used to detect the proportion of aortic atherosclerotic plaque deposition area, Ki67 and α-SMA expression. CCK-8, scratch test and Western blotting were used to analyze the proliferation and migration of mouse vascular smooth muscle cells, and the changes of adenosine monophosphate dependent protein kinase (AMPK), extracellular regulated protein kinase (ERK) pathway and cytoskeleton protein Pdlim5. ResultsCompared with model group, the proportion of aortic atherosclerotic plaque deposition, the number of positive cells of Ki67 and α-SMA, the proliferation and migration ability of vascular smooth muscle cells, and the ERK level decreased, while the AMPK and Pdlim5 levels increased (P＜0.05). ConclusionMetformin improves the abnormal proliferation of vascular smooth muscle cells and atherosclerosis in mice induced by high-fat diet by regulating AMPK and ERK pathways. |
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