| 李小鸽,陈聪,陈思睿,肖建华,梁柯嘉,刘彦.多肽ZLW调控日本血吸虫感染小鼠巨噬细胞极化改善肝纤维化.[J].中南医学科学杂志.,2023,(1):11-14. |
| 多肽ZLW调控日本血吸虫感染小鼠巨噬细胞极化改善肝纤维化 |
| The polypeptide ZLW regulates host macrophage polarization to improve liver fibrosis in schistosomiasis |
| 投稿时间：2022-05-24 修订日期：2022-11-29 |
| DOI：10.15972/j.cnki.43-1509/r.2023.01.003 |
| 中文关键词: 日本血吸虫 多肽ZLW 巨噬细胞 肝纤维化 [ |
| 英文关键词:schistosoma japonicum polypeptide ZLW macrophages liver fibrosis |
| 基金项目:国家自然科学基金(81101274)；湖南省创新平台开放基金项目(19K077) |
| 作者 | 单位 | E-mail | | 李小鸽 | 基础医学院病原研究所,
基础医学院寄生虫学教研室, | e-mail为1021494636@qq.com,e-mail为nhliuyan@126.com | | 陈聪 | 附属第一医院医学检验中心,湖南衡阳 421001 | | | 陈思睿 | 中国地质大学,湖北武汉 430000 | | | 肖建华 | 基础医学院病原研究所,
基础医学院寄生虫学教研室, | | | 梁柯嘉 | 基础医学院病原研究所,
基础医学院寄生虫学教研室, | | | 刘彦 | 基础医学院病原研究所,
基础医学院寄生虫学教研室, | e-mail为1021494636@qq.com,e-mail为nhliuyan@126.com |
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| 中文摘要: |
| 目的探究多肽ZLW对日本血吸虫感染小鼠巨噬细胞极化及肝纤维化的影响机制。 方法将24只BALB/c小鼠随机均分为对照组、模型组和ZLW组。模型组和ZLW组感染日本血吸虫尾蚴建立血吸虫肝纤维化模型。胶原纤维染色观察各组小鼠肝组织病理学改变；免疫组织化学染色观察肝脏巨噬细胞诱导型一氧化氮合酶(iNOS)、精氨酸酶1(Arg1)、CD68+巨噬细胞、CD206+巨噬细胞浸润情况；流式细胞术检测小鼠肝、脾细胞M2/M1变化；酶联免疫吸附试验法检测血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。 结果与模型组比较,ZLW组小鼠肝脏中纤维结缔组织增生减少、损伤减轻,CD206+细胞、Arg1、M2/M1、IL-6降低,而CD68+细胞、iNOS、TNF-α水平升高。 结论ZLW调控日本血吸虫感染小鼠巨噬细胞极化而改善肝纤维化。 |
| 英文摘要: |
| AimTo explore the effect of polypeptide ZLW on macrophage polarization and liver fibrosis in mice infected with schistosoma japonicum. Methods24 BALB/c mice were randomly divided into control group, model group and ZLW group. Schistosoma japonicum cercariae were infected in model group and ZLW group to establish schistosomiasis hepatic fibrosis model. The histopathological changes of liver were observed by collagen fiber staining. Immunohistochemical staining was used to observe the infiltration of inducible nitric oxide synthase (iNOS), arginase-1(Arg1), CD68+ macrophages and CD206+ macrophages in liver. Flow cytometry was used to detect the ratio changes of M2/M1 in liver and spleen cells of mice. Serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels were detected by enzyme-linked immunosorbent assay. ResultsCompared with the model group, the proliferation and damage of fibrous connective tissue in the liver of ZLW mice decreased, and the number of CD206+ cells, Arg1, M2/M1 and IL-6 decreased, while the levels of CD68+ cells, iNOS and TNF-α increased. ConclusionZLW regulates macrophage polarization in mice infected with schistosoma japonicum and improves liver fibrosis. |
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