| 杨剑波,曹倪豪,程飞,梁增亮,杨菲.土贝母苷甲通过调控miR-215-5p影响膀胱癌T24细胞增殖、迁移及侵袭.[J].中南医学科学杂志.,2022,(6):837-840. |
| 土贝母苷甲通过调控miR-215-5p影响膀胱癌T24细胞增殖、迁移及侵袭 |
| Effects of tubeimoside-1 on proliferation, migration and invasion of bladder cancer T24 cells by regulating the expression of miR-215-5p |
| 投稿时间：2021-12-08 修订日期：2022-08-28 |
| DOI：10.15972/j.cnki.43-1509/r.2022.06.013 |
| 中文关键词: 膀胱癌 土贝母苷甲 miR-215-5p 细胞增殖 迁移 侵袭 [ |
| 英文关键词:bladder cancer tubeimoside-1 miR-215-5p cell proliferation migration invasion |
| 基金项目:南通市海门区科技计划项目(2021SF18) 作者简介:杨剑波,主治医师,研究方向为泌尿系统疾病诊治,E-mail为yangjb0602@163.com。通信作者杨菲,主治医师,研究方向为泌尿外科临床研究,E-mail为69148982@qq.com。 |
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| 中文摘要: |
| 目的探讨土贝母苷甲通过调控miR-215-5p表达对膀胱癌T24细胞增殖、迁移及侵袭的影响。 方法不同质量浓度土贝母苷甲(10、20、30 mg/L)处理人膀胱癌细胞T24。细胞分别转染miR-NC、miR-215-5p mimics、anti-miR-NC、anti-miR-215-5p,随后用土贝母苷甲(30 mg/L)处理T24细胞。CCK-8实验检测细胞增殖；平板克隆形成实验检测细胞克隆形成能力；Transwell实验检测细胞迁移及侵袭；qRT-PCR法检测miR-215-5p的表达；Western blotting法检测基质金属蛋白酶-2(MMP-2)、MMP-9蛋白表达。 结果土贝母苷甲作用于T24细胞后,细胞存活率和MMP-2、MMP-9蛋白水平、细胞克隆形成、迁移及侵袭降低(P＜0.05),而miR-215-5p表达升高(P＜0.05),且呈剂量依赖性递减或递增。miR-215-5p过表达可抑制细胞增殖、克隆形成、迁移及侵袭(P＜0.05)；抑制miR-215-5p可逆转土贝母苷甲对T24细胞恶性行为的抑制作用。 结论土贝母苷甲可通过促进miR-215-5p的表达而抑制膀胱癌细胞恶性行为。 |
| 英文摘要: |
| To explore the effect of tubeimoside-1 on proliferation, migration and invasion of bladder cancer T24 cells by regulating the expression of miR-215-5p. MethodsDifferent concentrations of tubeimoside-1 (10,20, 30 mg/L) were used to treat human bladder cancer cells T24. miR-NC, miR-215-5p mimics, anti-miR-NC and anti-miR-215-5p were transfected, and then the T24 cells were treated with tubeimoside-1 (30 mg/L). CCK-8 experiment was used to detect cell proliferation. The plate clone formation test was used to detect the ability of cell clone formation. Transwell test detects cell migration and invasion. qRT-PCR method was used to detect the expression of miR-215-5p. Western blotting method was used to detect the protein expression of matrix metalloproteinases-2 (MMP-2) and MMP-9. ResultsAfter tubeimoside-1 acts on T24 cells, cell viability, protein levels of MMP-2, MMP-9, the formation, migration and invasion of cell clones decreased (P＜0.05), the expression of miR-215-5p increased (P＜0.05), and decreased or increased in a dose-dependent manner. Transfection of miR-215-5p mimics could inhibit cell proliferation, clone formation, migration and invasion (P＜0.05). Transfection of anti-miR-215-5p could reduce the inhibitory effects of tubeimoside-1 on the malignant behavior of T24 cells. ConclusionTubeimoside-1 could inhibit the malignant behavior of bladder cancer cells by promoting miR-215-5p expression. |
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