| 许诣,秦建品,沈文婷.人参皂苷通过NF-κB/NLRP3途径改善狼疮性肾炎小鼠肾损伤.[J].中南医学科学杂志.,2022,(6):832-836. |
| 人参皂苷通过NF-κB/NLRP3途径改善狼疮性肾炎小鼠肾损伤 |
| Ginsenoside through NF-κB/NLRP3 pathway improves renal injury in lupus nephritis mice |
| 投稿时间：2021-12-20 修订日期：2022-04-02 |
| DOI：10.15972/j.cnki.43-1509/r.2022.06.012 |
| 中文关键词: 人参皂苷 狼疮性肾炎 肾损伤 NF-κB/NLRP3通路 小鼠 [ |
| 英文关键词:ginsenosides lupus nephritis kidney damage NF-κB/NLRP3 pathway mice |
| 基金项目:湖北省卫生和计划生育委员会科研项目(WJ2017Q039) 作者简介:许诣,硕士,主治医师,研究方向为儿科疾病诊疗,E-mail为15971039756@163.com。通信作者沈文婷,硕士,主治医师,研究方向为儿科疾病诊疗,E-mail为sysysy135635@163.com。 |
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| 中文摘要: |
| 目的探究人参皂苷对狼疮性肾炎(LN)小鼠肾损伤及核因子-κB(NF-κB)/核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)通路的影响。方法将60只红斑狼仓小鼠随机均分为模型组、人参皂苷低、中、高剂量组、醋酸泼尼松组,另取12只C57BL/6小鼠为对照组。检测各组小鼠一般情况、肾功能指标；HE染色检测肾组织病理形态改变；免疫荧光染色检测肾组织IgG免疫复合物沉积；酶联免疫吸附法检测血清抗核抗体(ANA)、抗双链DNA(dsDNA)抗体含量及炎症因子白细胞介素-1β(IL-1β)、干扰素-γ(IFN-γ)含量；Western blotting检测肾组织NF-κB/NLRP3通路相关蛋白表达。结果与对照组比较,模型组小鼠精神萎靡、反应迟钝、活动减少、食欲减退、毛色暗淡,体质量明显减轻,肾组织发生明显病理损伤,24 h尿蛋白、血清肌酐(Cr)、ANA、抗dsDNA抗体、IL-1β及IFN-γ含量、肾组织IgG免疫荧光强度及NF-κB、NLRP3蛋白表达水平明显升高(P＜0.05)。与模型组比较,人参皂苷低、中、高剂量组及醋酸泼尼松组小鼠以上观察指标均得到明显改善,且人参皂苷各组指标随剂量递增而改善更明显(P＜0.05)。结论人参皂苷可下调NF-κB/NLRP3通路蛋白表达,抑制LN小鼠肾组织炎症反应,减轻IgG免疫复合物沉积,改善其肾功能。 |
| 英文摘要: |
| To investigate the effects of ginsenosides on renal injury and nuclear factor-κB(NF-κB)/NOD-like receptor protein 3 (NLRP3) pathway in lupus nephritis (LN) mice. MethodsSixty MRL/LPR mice were randomly divided into model group, ginsenoside low-dose, medium-dose and high-dose groups, prednisone acetate group, and 12 female C57BL/6 mice were set as control group. After drug grouping, the general condition, contents of renal function indexes of mice in each group were detected. The pathological and morphological changes of mouse kidney tissue were detected with hematoxylin-Eosin (HE) staining; the IgG immune complex deposition in mouse kidney tissue was detected with immunofluorescence staining; the contents of serum antinuclear antibody (ANA), anti-double-stranded DNA (dsDNA) antibody and inflammatory factors interleukin-1 β (IL-1β) and interferon-γ (IFN-γ)in mouse were detected with enzyme-linked immunosorbent assay; the expression of NF-κB/NLRP3 pathway related proteins in mouse kidney tissue was detected with Western blotting. ResultsCompared with the control group, the mice in the model group were depressed, unresponsive, with decreased activity, anorexia, dull hair color, and their body weight was significantly reduced, the kidney tissue had obvious pathological damage, the 24 h urine protein, serum creatinine (Cr), ANA, anti-dsDNA antibody, IL-1β and IFN-γ contents, kidney tissue IgG immunofluorescence intensity, and nuclear NF-κB and NLRP3 protein expression levels were significantly increased (P＜0.05). Compared with the model group, the above observation indexes of mice in the low, medium and high dosage groups of ginsenoside and prednisone acetate group were improved, and the indexes of each group of ginsenoside were improved more obviously with the increasing dosage (P＜0.05). ConclusionGinsenosides can down-regulate the expression of NF-κB/NLRP3 pathway proteins, inhibit the inflammation in the kidney tissue of LN mice, reduce the deposition of IgG immune complexes, and improve their kidney function. |
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