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薛小春,陆翠华,田尧.GLI4在结肠癌中的表达及对癌细胞生物学行为的影响.[J].中南医学科学杂志.,2022,(6):809-813.

GLI4在结肠癌中的表达及对癌细胞生物学行为的影响

Expression of GLI4 in colon cancer and its effect on the biological behavior of cancer cells

投稿时间：2022-01-06 修订日期：2022-08-07

DOI：10.15972/j.cnki.43-1509/r.2022.06.007

英文关键词:GLI4 colon cancer expression NK cells epithelial mesenchymal transformation dry

基金项目:国家自然科学基金项目(81071966)；湖南省教育厅科学研究项目(19A442)；湖南省大学生创新创业训练计划项目(S202110555312)；南华大学大学生创新创业训练计划项目(X202110555532,X202110555535) 作者简介:凌晖,博士,教授,硕士研究生导师,研究方向为非编码RNA与胃癌,E-mail为nhdxlh@126.com。

作者	单位	E-mail
薛小春	南通大学医学院,江苏省南通市226001 南通大学附属医院消化内科,江苏省南通市226001	e-mail为1253998688@qq.com
陆翠华	南通大学医学院,江苏省南通市226001 南通大学附属医院消化内科,江苏省南通市226001
田尧	海安市人民医院,江苏省海安市226600

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中文摘要:

目的探讨GLI4在结肠癌中的表达及对癌细胞生物学行为的影响。方法使用TCGA数据库对GLI4的差异表达进行分析和验证。行Kaplan-Meier和log-rank检验以评估不同GLI4水平对结肠癌患者中位生存期的影响。纳入结肠癌患者160例,免疫组织化学染色检测GLI4在癌组织和癌旁正常组织中的表达水平,转录组测序和免疫浸润分析GLI4对免疫细胞的潜在影响。过表达或敲低GLI4后,分析其对结肠癌细胞LoVo和SW1116增殖、上皮间充质转化能力和干性的影响。结果GLI4在结肠癌组织中的表达显著高于癌旁正常组织(P＜0.05)；GLI4高表达结肠癌患者中位生存期低于低表达患者,GLI4表达与淋巴结转移和分期具有相关性(P＜0.05)。过表达GLI4后,结肠癌细胞LoVo和SW1116增殖、上皮间充质转化能力和干性水平上升(P＜0.05)。CD56^bright NK细胞数与GLI4呈正相关。结肠癌细胞LoVo和SW1116中CD56^bright NK细胞抑制因子IL-4、IL-5、IL-6、IL-10在过表达GLI4后上升,在敲低GLI4后下降。结论高GLI4表达与结肠癌患者癌症进展和中位生存期显著相关。GLI4可以促进结肠癌细胞的增殖水平、上皮间充质转化能力和干性水平。

英文摘要:

To investigate the expression of GLI4 in colon cancer and its effect on the biological behavior of cancer cells. MethodsThe differential expression of GLI4 was analyzed and validated by using the TCGA database.Kaplan-Meier and log-rank tests were performed to assess the effect of different GLI4 levels on the survival rate of colon cancer patients. 160 colon cancer patients who admitted to our hospital from October 2019 to October 2021 were enrolled.Immunohistochemical staining was used to detect GLI4 expression levels in cancer and normal tissues, and transcriptome sequencing and immune infiltration were used to analyze the potential effects of GLI4 on immune cells. The effects of GLI4 overexpression or knockdown on the proliferation, epithelial mesenchymal transformation and dryness of colon cancer cells LoVo and SW1116 were analyzed. ResultsThe expression of LI4 was significantly higher in colon cancer tissues than normal tissues adjacent to the cancer (P＜0.05); the median survival of patients with high GLI4 expression in colon cancer was lower than who with low expression, and GLI4 expression correlated with lymph node metastasis and staging (P＜0.05). The proliferation, epithelial mesenchymal transition ability and stemness level of colon cancer cells LoVo and SW1116 increased after overexpression of GLI4 (P＜0.05). CD56^bright NK cell number were positively correlated with GLI4. CD56^bright NK cell suppressors IL-4, IL-5, IL-6, IL-10 in colon cancer cells LoVo and SW1116 increased after overexpression of GLI4 and decreased after knockdown of GLI4. ConclusionHigh GLI4 expression was significantly associated with cancer progression and median survival in colon cancer patients. GLI4 can promote the proliferation, epithelial mesenchymal transformation and dryness of colon cancer cells.

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