| 符国宏,赵宇青,郑杨慈,吴开李,李立新,王连臣.miR-299-3p靶向OCT4B1调控结直肠癌细胞SW480增殖、迁移、侵袭及凋亡.[J].中南医学科学杂志.,2022,(6):801-808, 822. |
| miR-299-3p靶向OCT4B1调控结直肠癌细胞SW480增殖、迁移、侵袭及凋亡 |
| miR-299-3p targets OCT4B1 to regulate the proliferation, migration, invasion and apoptosis of colorectal cancer cells SW480 |
| 投稿时间：2021-12-20 修订日期：2022-09-20 |
| DOI：10.15972/j.cnki.43-1509/r.2022.06.005 |
| 中文关键词: miR-299-3p OCT4B1 结直肠癌 细胞增殖 细胞迁移 细胞侵袭 细胞凋亡 [ |
| 英文关键词:miR-299-3p OCT4B1 colorectal cancer cell proliferation cell migration cell invasion apoptosis |
| 基金项目:海南省卫生厅科学研究课题(2002320271A2001) 作者简介:符国宏,副主任医师,研究方向为胃肠道肿瘤的诊治,E-mail为shanshanu1807@163.com。 |
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| 中文摘要: |
| 目的探讨miR-299-3p靶向调控OCT4B1对结直肠癌细胞SW480增殖、迁移、侵袭及凋亡的作用。 方法将SW480细胞分为NC组、mimics组、inhibitor组,采用MTT法检测细胞活力,结晶紫染色检测单克隆形成数,流式细胞术检测细胞凋亡,Transwell实验检测细胞侵袭能力,qRT-PCR测定细胞miR-299-3p和OCT4B1 mRNA,蛋白免疫印迹法测定OCT4B1蛋白表达。荧光素酶报告基因检测miR-556-3p对SW480细胞OCT4B1活性的调控作用。 结果与NC组比较,miR-299-3p水平、细胞凋亡率mimics组升高,inhibitor组降低,且mimics组高于inhibitor组(P＜0.05)；OCT4B1 mRNA和蛋白水平、细胞穿膜数、细胞存活率、单克隆形成数mimics组降低,inhibitor组升高,且mimics组低于inhibitor组(P＜0.05)。 结论miR-299-3p过表达可抑制结直肠癌细胞SW480增殖、迁移、侵袭,促进其凋亡,可能与miR-299-3p抑制OCT4B1 mRNA和蛋白表达有关。 |
| 英文摘要: |
| To investigate the effect of miR-299-3p on the proliferation, migration, invasion and apoptosis of colorectal cancer cells SW480 by targeting the regulation of OCT4B1. MethodsSW480 cells were divided into NC group, mimics group and inhibitor group. MTT assay was used to detect cell viability, crystal violet staining to detect monoclonal formation, flow cytometry to detect cell apoptosis, Transwell assay to detect cell invasion ability, qRT-PCR The miR-299-3p and OCT4B1 mRNA in cells were determined, and the protein expression of OCT4B1 was determined by western blotting. Luciferase reporter gene was used to detect the regulation of miR-556-3p on OCT4B1 activity in SW480 cells. ResultsCompared with the NC group, the level of miR-299-3p and the apoptosis rate of the mimics group were increased, while those in the inhibitor group were decreased, and the mimics group was higher than the inhibitor group (P＜0.05). The cell viability and the number of monoclonal formation in mimics group decreased, but increased in inhibitor group, and the mimics group was lower than the inhibitor group (P＜0.05). ConclusionmiR-299-3p overexpression can inhibit the proliferation, migration and invasion of colorectal cancer cells SW480, and promote their apoptosis, which may be related to the inhibition of OCT4B1 mRNA and protein expression by miR-299-3p. |
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