| 唐凤英,董汾,曾玉婷,高二鹏,张锋利,闫娣.miR-203a-3p通过ALOX15途径抑制胃癌细胞铁死亡.[J].中南医学科学杂志.,2022,(6):796-800. |
| miR-203a-3p通过ALOX15途径抑制胃癌细胞铁死亡 |
| miR-203a-3p inhibits iron death in gastric cancer cells via the ALOX15 pathway |
| 投稿时间：2021-12-22 修订日期：2022-08-20 |
| DOI：10.15972/j.cnki.43-1509/r.2022.06.004 |
| 中文关键词: miR-203a-3p ALOX15 胃癌细胞 铁死亡 [ |
| 英文关键词:miR-203A-3p ALOX15 gastric cancer cells ferroptosis |
| 基金项目:陕西省科技计划项目(2020XKTD-A01) 作者简介:唐凤英,副主任医师,研究方向为胃癌基础和临床研究,E-mail为xch.sx@163.com。通信作者董汾,硕士,主治医师,研究方向为胃癌基础和临床研究,E-mail为360154741@qq.com。 |
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| 中文摘要: |
| 目的探讨miR-203a-3p通过ALOX15途径调控胃癌细胞铁死亡的机制。 方法检测胃癌组织和癌旁正常组织的铁死亡指标——活性氧诱导脂质过氧化(lipid-ROS)水平。对胃癌组织miR-203a-3p与lipid-ROS进行Pearson相关性分析。在胃正常细胞和胃癌细胞株中过表达或敲低相关分子后,检测其lipid-ROS和线粒体膜电位(MMP)。 结果miR-203a-3p水平胃癌组织高于癌旁正常组织,胃癌细胞株高于胃正常细胞；lipid-ROS水平胃癌组织低于癌旁正常组织,胃癌细胞株低于胃正常细胞(P＜0.05)。在胃癌组织中,miR-203a-3p与lipid-ROS呈负相关(P＜0.05)。胃癌组织ALOX15 mRNA低于癌旁正常组织(P＜0.05)。过表达miR-203a-3p后,胃正常细胞lipid-ROS、胃癌细胞MMP、所有细胞ALOX15 mRNA和蛋白水平降低；敲低miR-203a-3p后,胃癌细胞lipid-ROS、所有细胞ALOX15 mRNA和蛋白升高；敲低ALOX15时胃正常细胞lipid-ROS和胃癌细胞MMP降低,过表达ALOX15后胃癌细胞lipid-ROS升高(P＜0.05)。 结论胃癌细胞高表达的miR-203a-3p可靶向ALOX15,减少ALOX15表达,抑制其铁死亡发生。 |
| 英文摘要: |
| To investigate the mechanism by which miR-203a-3p regulates iron death in gastric cancer cells through the ALOX15 pathway. MethodsThe levels of iron death indicators (reactive oxygen species-induced lipid peroxidation (lipid-ROS)) were detected in gastric cancer tissues and normal tissues adjacent to the cancer. Pearson correlation analysis was performed between miR-203a-3p and lipid-ROS in gastric cancer tissues. The lipid-ROS and mitochondrial membrane potential (MMP) were examined after overexpression or knockdown of the related molecules in gastric normal cells and gastric cancer cell lines. ResultsThe level of miR-203a-3p in gastric cancer tissues was higher than that in normal adjacent tissues, and the level of miR-203a-3p in gastric cancer cell lines was higher than that in normal gastric cells; Lipid ROS level in gastric cancer tissues was lower than that in normal adjacent tissues, and gastric cancer cell lines were lower than that in normal gastric cells (P＜0.05). In gastric cancer tissues, miR-203a-3p was negatively correlated with lipid-ROS (P＜0.05). ALOX15 mRNA in gastric cancer was lower than that in normal adjacent tissues (P＜0.05). After overexpression of miR-203a-3p, the level of lipid-ROS in normal gastric cells, MMP in gastric cancer cells, and ALOX15 mRNA and protein in all cells decreased; knock down miR-203a-3p, the lipid-ROS of gastric cancer cells, ALOX15 mRNA and protein of all cells increased; lipid-ROS in normal gastric cells and MMP in gastric cancer cells were decreased when ALOX15 was knocked down, while lipid-ROS in gastric cancer cells was increased after overexpression of ALOX15 (P＜0.05). ConclusionHigh expression of miR-203a-3p in gastric cancer cells targets ALOX15, reduces ALOX15 expression and inhibits iron death occurrence. |
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