| 钟轩,王红钰,高建超,赵金来,张诏然.木犀草素通过CTGF/EGFR通路对人结肠癌细胞LoVo增殖和凋亡的影响.[J].中南医学科学杂志.,2022,(4):486-490. |
| 木犀草素通过CTGF/EGFR通路对人结肠癌细胞LoVo增殖和凋亡的影响 |
| Effects of luteolin on proliferation and apoptosis of human colon cancer cells LoVo via CTGF/EGFR pathway |
| 投稿时间：2021-07-01 修订日期：2022-04-13 |
| DOI：10.15972/j.cnki.43-1509/r.2022.04.005 |
| 中文关键词: 木犀草素 结肠癌 细胞增殖 细胞凋亡 CTGF/EGFR通路 |
| 英文关键词:luteolin colon cancer cell proliferation apoptosis CTGF/EGFR pathway |
| 基金项目:河北省医学科学研究课题(20191341) 作者简介:钟轩,副主任医师,研究方向为胃肠肿瘤疾病诊治,E-mail为1127973696@qq.com。 |
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| 中文摘要: |
| 目的探讨木犀草素通过结缔组织生长因子(CTGF)/表皮生长因子受体(EGFR)通路对人结肠癌细胞LoVo增殖和凋亡的影响。方法MTT法确定木犀草素半数抑制质量浓度、作用时间。将细胞实验分为阴性对照组、阳性对照组、木犀草素低、中、高剂量组。阴性对照组细胞不进行处理,阳性对照组用40 μg/L西妥昔单抗干预,木犀草素低、中、高剂量组分别用20、40、80 μg/L木犀草素干预。采用流式细胞术检测各组细胞凋亡率,蛋白印迹法检测细胞CTGF、EGFR、蛋白质丝氨酸苏氨酸激酶(Akt)、B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸天冬氨酸酶3(Caspase-3)蛋白水平。结果MTT法确定木犀草素半数抑制质量浓度40 μg/L,半数抑制时间24 h。随着木犀草素质量浓度增加,LoVo细胞增殖率降低(P＜0.05)。与阴性对照组比较,其余各组细胞凋亡率、Bax和Caspase-3蛋白均增加,细胞CTGF、p-EGFR/EGFR、Akt和Bcl-2蛋白均降低(P＜0.05)。与阳性对照组比较,木犀草素各剂量组细胞凋亡率、Bax和Caspase-3蛋白均降低,细胞CTGF、p-EGFR/EGFR、Akt和Bcl-2蛋白均增加,且随木犀草素剂量增加呈剂量效应关系(P＜0.05)。结论木犀草素抑制LoVo细胞增殖,诱导LoVo细胞凋亡,其机制可能与抑制CTGF/EGFR通路的激活有关。 |
| 英文摘要: |
| To investigate the effect of luteolin on the proliferation and apoptosis of human colon cancer cell LoVo via connective tissue growth factor(CTGF)/epidermal growth factor receptor(EGFR) pathway. MethodsMTT method was used to determine the half inhibitory concentration and action time of luteolin. The cell was divided into negative control group, positive control group, low-dose luteolin group, middle-dose luteolin group and high-dose luteolin group. Negative control group was not treated, positive control group was treated with 40 μg/L cetuximab, luteolin low-dose, medium-dose and high-dose groups were treated with 20,40, 80 μg/L luteolin, respectively. The apoptosis rate of each group was detected by flow cytometry, and the protein levels of CTGF, EGFR, Serine threonine kinase (Akt), Bcl-2, Bcl-2 associated X protein (Bax) and cysteine aspartase 3 (Caspase-3) were detected by western blotting. ResultsMTT method was used to determine the median inhibitory concentration of luteolin 40 μg/L, 24 h as the median inhibitory time. With the increase of luteolin concentration, the proliferation rate of LoVo cells decreased(P＜0.05). Compared with negative control group, the apoptosis rate, Bax and Caspase-3 protein levels in the other groups were increased, and the CTGF, p-EGFR/EGFR, Akt and Bcl-2 protein levels decreased(P＜0.05). Compared with positive control group, the apoptosis rate, Bax and Caspase-3 protein levels in the all dose luteolin group were decreased, and the levels of CTGF, p-EGFR/EGFR, Akt and Bcl-2 protein increased, and the effect of luteolin dose groups presented a dose-response relationship with the increase of luteolin dose(P＜0.05). ConclusionLuteolin can inhibit LoVo cell proliferation and induce LoVo cell apoptosis, which may be related to inhibiting the activation of CTGF/EGFR pathway. |
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