| 田甜,韦海林,陆敦,陆岩.RDS合并呼吸机相关性肺炎新生儿肺部菌群多样性分析.[J].中南医学科学杂志.,2022,(3):417-420. |
| RDS合并呼吸机相关性肺炎新生儿肺部菌群多样性分析 |
| Analysis of pulmonary flora diversity in neonatal respiratory distress syndrome complicated with ventilator-associated pneumonia |
| 投稿时间：2021-06-12 修订日期：2021-11-23 |
| DOI：10.15972/j.cnki.43-1509/r.2022.03.027 |
| 中文关键词: 新生儿呼吸窘迫综合征 呼吸机相关性肺炎 肺部菌群 菌群丰度 |
| 英文关键词:neonatal respiratory distress syndrome ventilator-associated pneumonia pulmonary flora species richness |
| 基金项目:河池市科学技术局项目(河科AB200742) 作者简介:田甜,副主任医师,研究方向为新生儿疾病诊治,E-mail为318798079@qq.com。 |
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| 中文摘要: |
| 目的研究呼吸窘迫综合征(RDS)合并呼吸机相关性肺炎(VAP)新生儿的机械通气时间与其肺部菌群多样性之间的关系。方法选择行气管插管RDS新生儿70例,根据是否并发VAP分为VAP组和对照组。气管插管第1~5天采集两组患儿痰液标本,进行细菌DNA提取、PCR扩增以及变性梯度凝胶电泳试验,比较两组患儿肺部菌群丰度以及香农-维纳指数。结果VAP组患儿机械通气时间、拔管后吸氧时间、重复插管上机率及死亡率高于对照组(P＜0.05)。VAP组30例患儿中共检出36株菌株,以革兰氏阴性菌为主(72.22%),VAP组第2~4天肺部菌群丰度和香农-维纳指数均小于对照组(P＜0.05)。结论RDS患儿机械通气过程中VAP发生率高,机械通气过程中肺部菌群多样性降低可提示VAP的发生。 |
| 英文摘要: |
| To investigate the relationship between mechanical ventilation time and pulmonary flora diversity in neonatal respiratory distress syndrome (RDS) complicated with ventilator-associated pneumonia (VAP). MethodsSeventy neonates with RDS receiving endotracheal intubation and ventilation were enrolled, and divided into two groups according to the presence or absence of VAP, VAPgroup and control group. Sputum specimens were collected on the 1st to 5th day of endotracheal intubation (D1~D5) for bacterial DNA extraction, polymerase chain reaction (PCR) amplification and denaturing gradient gel electrophoresis (DGGE) test. Then the species richness and Shannon-Wiener index of two groups were compared. ResultsThe mechanical ventilation time, oxygen inhalation time after extubation, repetition intubation, rate mortality rate of VAP group was significantly higher than that of control group (P＜0.05).A total of 36 strains were isolated in VAP group, which were dominated by Gram-negative bacteria (72.22%). The species richness and Shannon-Wiener index at D2~D4 were significantly lower in VAP group than in control group, with statistical difference (all P＜0.05). ConclusionNeonates with RDS are at high risk of VAP during mechanical ventilation, and a reduction in pulmonary flora diversity during mechanical ventilation may suggest the development of VAP. |
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