| 静亮,彭希,张友平,李大勇,石迎花,祝伟.mNGS在脓毒症病原体检测和抗生素治疗中的应用.[J].中南医学科学杂志.,2022,(3):367-369. |
| mNGS在脓毒症病原体检测和抗生素治疗中的应用 |
| Application of mNGS in sepsis pathogen detection and antibiotic treatment |
| 投稿时间：2021-10-02 修订日期：2021-12-04 |
| DOI：10.15972/j.cnki.43-1509/r.2022.03.013 |
| 中文关键词: 宏基因组学下一代测序 脓毒症 病原体 抗生素 |
| 英文关键词:metagenomics next generation sequencing sepsis pathogen antibiotics |
| 基金项目:湖北省卫生计生委科研项目(WJ2019M123) 作者简介:静亮,博士,主治医师,研究方向为脓毒症和脑血管病的治疗,E-mail为133123311@qq.com。通信作者祝伟,博士,主任医师,研究方向为脓毒症和脑血管病的治疗,E-mail为tjjzkzw512@163.com。 |
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| 中文摘要: |
| 目的分析宏基因组学下一代测序(mNGS)在脓毒症患者病原体检测和抗生素应用中的临床价值,为疾病快速、准确诊断和改善患者临床预后提供新型诊断技术。方法选择确诊脓毒症患者52例,入院24 h内完成样本(血液、尿液、痰液或者肺泡灌洗液)收集,分别采用传统技术(细菌培养和免疫学方法)和mNGS法进行病原体检测。结果mNGS法诊断病原体的时间比传统法明显缩短,识别率提高,准确率提高(P＜0.05)。根据传统技术建议首次采用广谱抗生素,而根据mNGS则推荐使用针对性更强的高效抗生素。结论脓毒症患者采用mNGS法能够缩短病原体检测时间,提高病原体识别率和准确率,可作为指导抗生素应用的依据。 |
| 英文摘要: |
| To analyze the clinical value of metagenomics next generation sequencing (mNGS) in pathogen detection and antibiotic application in patients with sepsis, so as to provide a new diagnostic technology for rapid and accurate diagnosis of the disease and improving the clinical prognosis of patients. Methods52 patients with sepsis diagnosed in intensive care unit were selected. Samples (blood, urine, sputum or alveolar lavage fluid) were collected within 24 hours after admission. Pathogens were detected by traditional techniques (bacterial culture and immunological methods) and mNGS respectively. ResultsThe time of diagnosing pathogens in mNGS group was significantly shorter than that in traditional group, and the recognition rate and accuracy were improved (P＜0.05). According to the traditional technology, broad-spectrum antibiotics are recommended for the first time, while more targeted and efficient antibiotics are recommended according to mNGS. ConclusionmNGS in patients with sepsis can further shorten the pathogen detection time, improve the pathogen recognition rate and accuracy, and serve as the basis for guiding the application of antibiotics. |
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