| 刘丽娜,杨锋.基于生物信息学及网络药理学分析丹皮酚抗瘢痕疙瘩的作用机制.[J].中南医学科学杂志.,2022,(3):362-366. |
| 基于生物信息学及网络药理学分析丹皮酚抗瘢痕疙瘩的作用机制 |
| Analysis of anti keloid mechanism of paeonol based on bioinformatics and network pharmacology |
| 投稿时间：2021-04-29 修订日期：2021-10-22 |
| DOI：10.15972/j.cnki.43-1509/r.2022.03.012 |
| 中文关键词: 丹皮酚 瘢痕疙瘩 生物信息学 网络药理学 |
| 英文关键词:paeonol keloid bioinformatics network pharmacology |
| 基金项目:湖南省卫生健康委员会科研计划课题项目(20191237) 作者简介:刘丽娜,硕士研究生,研究方向为瘢痕畸形修复及瘢痕疙瘩的治疗,E-mail为1072662286@qq.com。通信作者杨锋,硕士,副教授,硕士研究生导师,研究方向为眼整形修复及瘢痕治疗等,E-mail为103549245@qq.com。 |
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| 中文摘要: |
| 目的筛选丹皮酚作用于瘢痕疙瘩(KD)的潜在药物靶点和信号通路,初步分析丹皮酚抗KD的作用机理。方法从CTD和GeneCards数据库中获取KD致病基因,从TCMSP和PubChem数据库中获取丹皮酚直接靶标基因。采用STRING数据库和Cytoscape3.7.1软件进行网络拓扑分析,筛选出KD候选基因、丹皮酚候选靶点基因和丹皮酚的潜在药物靶点。利用DIVAD软件进行基因功能富集(GO)分析和信号通路富集(KEGG)分析,并采用Cytoscape软件构建药物靶点网络图。下载GEO芯片数据进行最佳药物靶点验证。结果共筛选出KD候选基因143个、丹皮酚候选靶点基因29个,丹皮酚抗KD的潜在药物靶点11个,并构建出一个包含11个节点、86条边的潜在药物靶点PPI网络；这些潜在药物靶点与磷脂腺肌醇3激酶/蛋白质丝氨酸苏氨酸激酶(PI3K/Akt)信号通路、凋亡信号通路及核转录因子-κB(NF-κB)信号通路等相关。结论丹皮酚可能通过作用于PI3K/Akt信号通路、凋亡通路及NF-κB信号通路中的核转录因子κB抑制剂、哺乳动物雷帕霉素靶蛋白、B细胞淋巴瘤-2、磷脂酶和张力蛋白同源物、转化生长因子β1、Akt1、丝裂原活化蛋白激酶1、白细胞介素-6、NF-κB1、纤维连接蛋白1、半胱氨酸蛋白酶3起到抗KD的作用。 |
| 英文摘要: |
| To screen the potential drug targets and signal pathways of paeonol on keloid (KD), and to analyze the anti-keloid mechanism of paeonol. MethodsThe pathogenesis related genes of KD were obtained from CTD and GeneCards databases, and the direct target genes of paeonol were obtained from TCMSP and PubChem databases. Using STRING database and Cytoscape 3.7.1 software for network topology analysis, keloid candidate genes, paeonol candidate target genes and potential drug targets were screened. DIVAD software was used to analyze the gene GO function and KEGG signal pathway enrichment, and Cytoscape software was used to construct the drug targets network diagram. Download GEO chip data to verify the best drug targets. Results143 candidate genes for keloid, 29 candidate target genes for paeonol and 11 potential drug targets for paeonol against keloid were obtained, and a potential drug target PPI network with 11 nodes and 86 edges was constructed; These potential drug targets are related to Phosphatidylinositol 3 kinase/protein kinase B(PI3K/Akt)signaling pathway, apoptosis signaling pathway and Nuclear transcription factor-κB(NF-κB)signaling pathway. ConclusionPaeonol may play an anti-keloid role by targeting on nuclear transcription factor kB inhibitors, mammalian target of rapamycin, B-cell lymphoma -2, phosphatase and tensin homolog, transforming growth factor-beta 1, Akt1, mitogen-activated protein kinase 1, IL-6, NF-κB1, fibronectin, Caspase-3 in PI3K/Akt signaling pathway, apoptosis pathway and NF-κB signaling pathway, which may contribute to the study of the specific anti-keloid mechanism of paeonol. |
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