| 曹军营,崔素娟,刘高峰,汤光耀,张勇,丁小勇.lncRNA MNX1-AS1通过调控miR-218-5p影响非小细胞肺癌细胞的增殖、凋亡及迁移.[J].中南医学科学杂志.,2022,(3):336-340. |
| lncRNA MNX1-AS1通过调控miR-218-5p影响非小细胞肺癌细胞的增殖、凋亡及迁移 |
| LncRNA MNX1-AS1 affects the proliferation, apoptosis and migration of non-small cell lung cancer cells by regulating miR-218-5p |
| 投稿时间：2021-07-03 修订日期：2021-07-08 |
| DOI：10.15972/j.cnki.43-1509/r.2022.03.006 |
| 中文关键词: MNX1-AS1 miR-218-5p 非小细胞肺癌 增殖 迁移 |
| 英文关键词:MNX1-AS1 miR-218-5p non-small cell lung cancer proliferation migration |
| 基金项目:河南省医学科技攻关计划联合共建项目(LHGJ20190876) 作者简介:曹军营,主治医师,研究方向为肺癌、胸部外伤,E-mail为26792130@qq.com。通信作者刘高峰,副主任医师,研究方向为肺癌、食管癌、胸部外伤,E-mail为Liugfxw@163.com。 |
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| 中文摘要: |
| 目的探讨长链非编码RNA(lncRNA)MNX1-AS1通过调控miR-218-5p的表达对非小细胞肺癌(NSCLC)细胞增殖、凋亡和迁移的影响。方法qRT-PCR检测MNX1-AS1和miR-218-5p在肺癌细胞系及肺正常上皮细胞系中的表达；采用双荧光素酶报告基因实验和RNA pull down实验验证MNX1-AS1和miR-218-5p之间的靶向关系；干扰A549细胞中MNX1-AS1和miR-218-5p的表达后,MTT、流式细胞术和Transwell实验检测MNX1-AS1靶向miR-218-5p对癌细胞增殖、凋亡、迁移及侵袭的影响。结果与肺正常上皮细胞系相比,肺癌细胞系中MNX1-AS1表达升高,miR-218-5p表达降低(P＜0.05)；荧光素酶报告基因实验显示,MNX1-AS1可与miR-218-5p结合导致荧光素酶活性显著降低(P＜0.05)；RNA pull down实验证实miR-218-5p能特异性结合MNX1-AS1(P＜0.05)；抑制A549细胞中MNX1-AS1的表达后,miR-218-5p水平上调,细胞的增殖、迁移和侵袭能力均显著降低,凋亡率显著升高；与MNX1-AS1抑制剂组相比,共抑制MNX1-AS1和miR-218-5p的细胞增殖、迁移、侵袭能力升高,凋亡率下降(P＜0.05)。结论MNX1-AS1可以通过靶向下调miR-218-5p的表达影响非小细胞癌的增殖、凋亡、迁移和侵袭。 |
| 英文摘要: |
| To investigate the effect of long non-coding RNA (lncRNA) MNX1-AS1 regulating miR-218-5p expression on proliferation, apoptosis and migration of non-small cell lung cancer (NSCLC) cells. MethodsThe expressions of MNX1-AS1 and miR-218-5p in lung cancer cell lines and normal lung epithelial cell lines were detected by qRT-PCR. Double luciferase report assay and RNA pull down assay were used to verify the targeting relationship between MNX1-AS1 and miR-218-5p. After interfered with the expression of MNX1-AS1 and miR-218-5p in A549 cells, MTT, flow cytometry and Transwell assay detected the effect of MNX1-AS1-targeted miR-218-5p on the proliferation, apoptosis, migration and invasion of cancer cells. ResultsCompared with normal lung epithelial cell lines, the expression of MNX1-AS1 was increased and the expression of miR-218-5p was decreased in lung cancer cells (P＜0.05). Luciferase reporter gene assay showed that MNX1-AS1 could bind to miR-218-5p, resulting in a significant decrease in luciferase activity (P＜0.05); And the RNA pull down assay also confirmed that miR-218-5p could specifically bind to MNX1-AS1(P＜0.05). After inhibiting the expression of MNX1-AS1 in A549 cells, miR-218-5p was up-regulated, cell proliferation, migration and invasion ability were significantly reduced, and apoptosis rate was significantly increased. Compared with the MNX1-AS1 inhibitor group, the cell proliferation, migration and invasion ability were increased, and the apoptosis rate was decreased after co-inhibited MNX1-AS1 and miR-218-5p (P＜0.05). ConclusionMNX1-AS1 can affect the proliferation, apoptosis, migration and invasion of non-small cell carcinoma by down-regulating the expression of miR-218-5p. |
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