| 廖年春,张潇迪,刘珏.miR-200b与miR-200c靶向DNA甲基转移酶对卵巢癌细胞顺铂耐药性的影响.[J].中南医学科学杂志.,2022,(2):190-193. |
| miR-200b与miR-200c靶向DNA甲基转移酶对卵巢癌细胞顺铂耐药性的影响 |
| Effects of miR-200b and miR-200c targeted DNA methyltransferases on cisplatin resistance in ovarian cancer cells |
| 投稿时间：2021-01-22 修订日期：2021-12-06 |
| DOI：10.15972/j.cnki.43-1509/r.2022.02.008 |
| 中文关键词: miR-200b miR-200c DNA甲基转移酶 卵巢癌 顺铂 耐药 |
| 英文关键词:miR-200b miR-200c DNA methyltransferase ovarian cancer cisplatin drug resistance |
| 基金项目:湖南省临床医疗技术创新引导计划(2018SK51509)；湖南省卫生健康委科研课题(B2019115) 作者简介:廖年春,硕士研究生,研究方向为妇科肿瘤的治疗,E-mail为2523712454@qq.com。通信作者刘珏,硕士,主任医师,硕士研究生导师,研究方向为妇科肿瘤学的治疗,E-mail为lj3457@163.com。 |
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| 中文摘要: |
| 目的探讨miR-200b与miR-200c靶向DNA甲基转移酶1(DNMT1)、DNMT3A和DNMT3B对卵巢癌细胞顺铂耐药性的影响。方法以人卵巢癌细胞株(A2780)、人卵巢癌顺铂耐药细胞株(A2780/DDP)作为研究对象,实时荧光定量PCR(qRT-PCR)检测A2780、A2780/DDP细胞株中miR-200b与miR-200c表达量。耐药细胞株A2780/DDP转染后分为si-miR-200b组、si-miR-200c组、si-NC组,MTT法检测转染后3组细胞IC50值；蛋白印迹免疫法(Western blotting)检测转染后3组细胞DNMT1、DNMT3A和DNMT3B蛋白表达水平；流式细胞术检测转染后细胞凋亡率。结果qRT-PCR结果显示A2780细胞系中miR-200b与miR-200c表达高于A2780/DDP细胞系(P＜0.05)。 si-miR-200b组和si-miR-200c组IC50值、DNMT1、DNMT3A 和DNMT3B 蛋白表达水平低于si-NC组(P＜0.05)。结论miR-200b与miR-200c高表达能抑制DNMT1、DNMT3A和DNMT3B基因表达,从而逆转卵巢癌细胞对顺铂的耐药性。 |
| 英文摘要: |
| To investigate the effects of miR-200b and miR-200c targeted DNA methyltransferase 1 (DNMT1), DNMT3A and DNMT3B on cisplatin resistance of ovarian cancer cells. MethodsHuman ovarian cancer cell line (A2780) and human ovarian cancer cisplatin-resistant cell line (A2780/DDP) were used as research objects, and the expression levels of miR -200b and miR -200C in A2780 and A2780/DDP cells were detected by real-time quantitative PCR (qRT-PCR). Drug-resistant cell line A2780/DDP were transfected into si-miR -200b group, si-miR -200c group and si-NC group. IC50 values of the three groups were detected by MTT assay. Western blotting was used to detect the protein expression levels of DNMT1, DNMT3A and DNMT3B after transfection. The apoptosis rate of transfected cells was detected by flow cytometry. ResultsqRT-PCR showed that the expression of miR-200b and miR-200c in A2780 cell line was higher than that in A2780/DDP cell line (P＜0.05). The IC50 value and protein expression levels of DNMT1, DNMT3A and DNMT3B in si-miR-200b and si-miR-200C groups were lower than those in si-NC group (P＜0.05). ConclusionHigh expression of miR-200b and miR-200c can inhibit the expression of DNMT1, DNMT3A and DNMT3B genes, thereby reversing the resistance of ovarian cancer cells. |
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