| 李柱,吴烜,彭小丹,王树滨.miR-145通过mTOR途径调控NSCLC A549细胞生物学特性.[J].中南医学科学杂志.,2022,(2):179-183. |
| miR-145通过mTOR途径调控NSCLC A549细胞生物学特性 |
| miR-145 regulates the biological characteristics of NSCLC A549 cells through mTOR pathway |
| 投稿时间：2021-06-24 修订日期：2021-12-09 |
| DOI：10.15972/j.cnki.43-1509/r.2022.02.006 |
| 中文关键词: miR-145 mTOR途径 非小细胞肺癌 A549细胞 生物学特性 |
| 英文关键词:miR-145 mTOR pathway non-small cell lung cancer A549 cells biological characteristics |
| 基金项目:深圳市科技计划项目(ZDSYS20190902092855097) 作者简介:李柱,副主任医师,研究方向为胃肠道肿瘤和肺癌的诊治,E-mail为563904040@qq.com。通信作者王树滨,博士,主任医师,研究方向为肿瘤化疗耐药与逆转,E-mail为wangshubin2013@163.com。 |
|
| 摘要点击次数: 352 |
| 全文下载次数: 243 |
| 中文摘要: |
| 目的探究miR-145调控非小细胞肺癌(NSCLC)A549细胞生物学特性及对雷帕霉素靶蛋白(mTOR)信号转导通路的影响。方法将A549细胞分为miR-145组、对照组、miR-145抗体组和对照抗体组。采用LipofectamineTM 2000转染试剂盒进行质粒转染,行MTT、细胞凋亡、细胞迁移、细胞侵袭检测,Western blotting检测分析雷帕霉素靶蛋白(mTOR)、磷酸化mTOR(p-mTOR)、真核细胞翻译起始因子(eIF4E)、磷酸化eIF4E(p-eIF4E)、核糖体蛋白S6激酶(p70S6K)、磷酸化p70S6K(p-p70S6K)、S6核糖体蛋白(S6)、磷酸化S6(p-S6)、eIF4E结合蛋白1(4E-BP)、磷酸化4E-BP(p-4E-BP)水平。结果对照组和对照抗体组细胞miR-145表达和细胞凋亡水平差异无显著性(P＞0.05),但与miR-145组和miR-145抗体组比较有明显差异,其中miR-145组最高,miR-145抗体组最低(P＜0.05)。对照组和对照抗体组细胞增殖、迁移、侵袭活性,以及p-mTOR/mTOR、p-eIF4E/eIF4E、p-p70S6K/p70S6K、p-S6/S6、p-4E-BP/4E-BP表达水平差异无显著性(P＞0.05),但与miR-145组和miR-145抗体组比较有明显差异,其中miR-145组最低,miR-145抗体组最高(P＜0.05)。结论miR-145可通过mTOR途径调控NSCLC A549细胞生物学特性,促进细胞凋亡,抑制细胞增殖活性、迁移能力及侵袭能力。 |
| 英文摘要: |
| To analyze the cell biological characteristics of miR-145 regulating non-small-cell lung cancer (NSCLC) A549 and its effect on mammalian target of rapamycin (mTOR) signal transduction pathway. MethodsA549 cells were divided into miR-145 group, control group, miR-145+antagonists group and control+antagonists group. The cells were transfected with plasmid by LipofectamineTM 2000 transfection kit, and were detected by MTT, apoptosis test, migration test and invasion test. The levels ofmammalian target of rapamycin (mTOR), phosphorylated mTOR (p-mTOR), eukaryotic initiation factor-4E (eIF4E), phosphorylated eIF4E (p-eIF4E), ribosomal protein S6 kinase (p70S6K), phosphorylated p70S6K (p-p70S6K), ribosomal protein S6 (S6), phosphorylated S6 (p-S6), eIF4E-binding protein (4E-BP), phosphorylated 4E-BP (p-4E-BP)were detected by Western blotting. ResultsThe miR-145 expression levels and cell apoptosis levels between the control group and control+antagonists group showed no significant difference (P＞0.05), however, which significantly differed with the miR-145 group and miR-145+antagonists group, and the miR-145 group was the highest, the miR-145+antagonists group was the lowest, and the difference was statistically significant (P＜0.05). The cell proliferation, cell migration, cell invasion activities and the expression levels of p-mTOR/mTOR, p-eIF4E/eIF4E, p-p70S6K/p70S6K, p-S6/S6, p-4E-BP/4E-BP between the control group and control+antagonists group showed no significant difference (P＞0.05), however, which significantly differed with the miR-145 group and miR-145+antagonists group, and the miR-145 group was the lowest, the miR-145+antagonists group was the highest, and the difference was statistically significant (P＜0.05). ConclusionmiR-145 can regulate the biological characteristics of NSCLC A549 cells through mTOR pathway, promote cell apoptosis, inhibit cell proliferation, migration and invasion. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|