| 闫凯欣,徐霄,补娟,徐红.卡维地洛对ox-LDL诱导内皮细胞中NLRP3炎症小体介导焦亡及炎症的影响.[J].中南医学科学杂志.,2022,(2):171-174. |
| 卡维地洛对ox-LDL诱导内皮细胞中NLRP3炎症小体介导焦亡及炎症的影响 |
| Effects of carvedilol on ox-LDL induced NLRP3 inflammasome mediated pyroptosis and inflammation of endothelial cell |
| 投稿时间：2021-05-12 修订日期：2021-12-13 |
| DOI：10.15972/j.cnki.43-1509/r.2022.02.004 |
| 中文关键词: 卡维地洛 NLRP3炎症小体 氧化型低密度脂蛋白 内皮细胞 焦亡 炎症反应 |
| 英文关键词:carvedilol NLRP3 inflammasome ox-LDL endothelial cell pyroptosis inflammatory response |
| 基金项目:新疆维吾尔自治区自然科学基金项目(2019D01C113) 作者简介:闫凯欣,硕士研究生,研究方向为老年医学,E-mail为thchr632@163.com。 |
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| 中文摘要: |
| 目的研究卡维地洛对氧化型低密度脂蛋白(ox-LDL)诱导内皮细胞中NLRP3炎症小体介导焦亡及炎症的调控作用。方法人脐静脉内皮细胞(HUVEC)分为对照组、ox-LDL组、低剂量组(0.01 mmol/L)、高剂量组(0.1 mmol/L)；转染阴性对照腺病毒(Ad-NC)或过表达NLRP3腺病毒(Ad-NLRP3)后分为Ad-NC组、Ad-NC+ox-LDL组、Ad-NC+高剂量组、Ad-NLRP3+高剂量组。检测各组细胞活力和白细胞介素(IL)-1β、IL-18含量,以及GSDMD-N、NLRP3、Cleaved Caspase-1表达水平。 结果ox-LDL组细胞活力低于对照组,IL-1β、IL-18及GSDMD-N、NLRP3、Cleaved Caspase-1均高于对照组(P＜0.05)。低剂量组和高剂量组细胞活力高于ox-LDL组,IL-1β、IL-18及GSDMD-N、NLRP3、Cleaved Caspase-1均低于ox-LDL组(P＜0.05)。转染NLRP3腺病毒后,Ad-NLRP3+高剂量组和Ad-NC+ox-LDL组细胞活力低于Ad-NC+高剂量组,IL-1β、IL-18及GSDMD-N、NLRP3、Cleaved Caspase-1均高于Ad-NC+高剂量组(P＜0.05)。 结论卡维地洛显著抑制ox-LDL诱导HUVEC中NLRP3炎症小体介导的细胞焦亡及炎症反应。 |
| 英文摘要: |
| Aim To study the regulatory effect of carvedilol on oxidized low density lipoprotein(ox-LDL) induced NLRP3 inflammasome mediated pyroptosis and inflammation of endothelial cell.MethodsHuman umbilical vein endothelial cells (HUVEC) were divided into control group, ox-LDL group, low dose group (0.01 mmol/L) and high dose group (0.1 mmol/L). After transfection with negative control adenovirus (Ad-NC) or overexpressing NLRP3 adenovirus (Ad-NLRP3), HUVEC were divided into Ad-NC group, Ad-NC+ox-LDL group, Ad-NC+high dose group and Ad-NLRP3+high dose group. Cell viability and the contents of interleukin (IL)-1β, IL-18 and the expression levels of GSDMD-N, NLRP3 and Cleaved Caspase-1 were measured.ResultsThe cell viability in ox-LDL group was lower than that in control group, IL-1β、IL-18, GSDMD-n, NLRP3 and Cleaved Caspase-1 were higher than those in the control group (P＜0.05). The cell viability in low-dose and high-dose group were higher than that in ox-LDL group, IL-1β, IL-18, GSDMD-N, NLRP3, Cleaved Caspase-1 were lower than those in ox-LDL group (P＜0.05). After infection with NLRP3 adenovir, the cell viability in Ad-NLRP3+high-dose group and in Ad-NC+ox-LDL group were lower than that in Ad-NC+high-dose group, IL-1β, IL-18, GSDMD-N, NLRP3, Cleaved Caspase-1 were higher than those in Ad-NC+high-dose group(P＜0.05).ConclusionCarvedilol significantly inhibits ox-LDL induced NLRP3 inflammasome mediated pyroptosis and inflammatory response in HUVEC. |
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