| 苏波,杨咏梅,刘政海,何慧,苏琦.Adiporedoxin对TNFα诱导血管内皮细胞Integrin α4和Integrin β1表达的影响.[J].中南医学科学杂志.,2022,(2):167-170. |
| Adiporedoxin对TNFα诱导血管内皮细胞Integrin α4和Integrin β1表达的影响 |
| The effect of adiporedoxin on TNF-α-induced Integrin α4 and Integrin β1 expression in vascular endothelial cells |
| 投稿时间：2021-08-13 修订日期：2021-11-18 |
| DOI：10.15972/j.cnki.43-1509/r.2022.02.003 |
| 中文关键词: 脂过氧化物酶 肿瘤坏死因子-α 整合素α4 整合素β1 血管内皮细胞 |
| 英文关键词:adiporedoxin TNF-α Integrin α4 Integrin β1 vascular endothelial cells |
| 基金项目:国家自然科学基金(81973532)；湖南省自然科学基金(2020JJ4529,2020JJ4522)；博士科研启动基金(2018XQD11,2011XQD34) 作者简介:苏波,博士,副教授,研究方向为抗肿瘤药物作用机制的研究,E-mail为subosu123@163.com。 |
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| 中文摘要: |
| 目的研究脂过氧化物酶(adiporedoxin,Adrx)对肿瘤坏死因子-α(TNF-α)诱导血管内皮细胞整合素α4(Integrin α4)和整合素β1(Integrin β1)表达的影响。方法过表达实验分为空载体组、空载体+TNF-α组、Adrx+TNF-α组；si-RNA干扰实验分为si-Control组、TNF-α组、si-Adrx+TNF-α组。将Adrx质粒和si-Adrx分别转染HUVEC细胞后,用TNF-α处理细胞,Real-time PCR和Western blotting分别检测Integrin α4和Integrin β1 mRNA和蛋白水平。结果TNF-α上调Integrin α4、Integrin β1表达；与单用TNF-α处理组比较,Adrx+TNF-α组细胞Integrin α4和Integrin β1 mRNA和蛋白水平均显著降低；而用si-RNA下调Adrx表达后,TNF-α诱导的Integrin α4和Integrin β1 mRNA和蛋白水平均明显升高。结论Adrx负调控TNF-α诱导的HUVEC细胞Integrin α4和Integrin β1表达。 |
| 英文摘要: |
| To investigate the effect of adiporedoxin (Adrx) on Integrin α4 and Integrin β1 expression induced by tumor necrosis factor-α (TNF-α) in vascular endothelial cells. MethodsThe overexpression experimental group was divided into vector group, vector+TNF-α group, Adrx+TNF-α group. si-RNA interference experimental group was divided into si-Control group, TNF-α group, si-Adrx+TNF-α group. HUVEC cells were transfected with Adrx plasmid and Si-Adrx plasmid, and treated with TNF-α. The mRNA and protein levels of Integrin α4 and Integrin β1 were detected by real-time PCR and Western blotting, respectively. ResultsTNF-α upregulated Integrin α4 and Integrin β1 expression. In contrast to cell treated with TNF-α alone, the mRNA and protein levels of Integrin α4 and Integrin β1 were obviously decreased in the Adrx+TNF-α group. TNF-α-induced Integrin α4 and Integrin β1 mRNA and protein levels were significantly increased after the expression of Adrx was down-regulated by si-RNA. ConclusionAdrx can negatively modulates Integrin α4 and Integrin β1 expression induced by TNF-α in HUVEC cells. |
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