| 张扬,王文斌.罗米司亭对血小板减少性紫癜小鼠BMNC中Notch信号以及PBMC中GATA-3和PD-1表达的影响.[J].中南医学科学杂志.,2022,(1):59-62. |
| 罗米司亭对血小板减少性紫癜小鼠BMNC中Notch信号以及PBMC中GATA-3和PD-1表达的影响 |
| The effects of Romipristine on Notch signal of BMNC, GATA-3 and PD-1 of PBMC in mice with immune thrombocytopenic purpura |
| 投稿时间：2021-03-12 修订日期：2021-06-04 |
| DOI：10.15972/j.cnki.43-1509/r.2022.01.013 |
| 中文关键词: 血小板减少性紫癜 罗米司亭 Notch信号通路 GATA-3 程序性细胞死亡受体-1 小鼠 |
| 英文关键词:ITP Romiplostim Notch signaling pathway GATA-3 PD-1 mice |
| 基金项目:重庆市科卫联合医学科研项目(2018QNXM042) 作者简介:张扬,硕士,主治医师,研究方向为血液病诊断和治疗,E-mail为wenmeiz1812@163.com。 |
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| 中文摘要: |
| 目的探究罗米司亭对血小板减少性紫癜(ITP)小鼠骨髓单个核细胞(BMNC)中Notch信号通路,以及外周血单个核细胞(PBMC)中转录因子结合蛋白-3(GATA-3)和程序性细胞死亡受体-1(PD-1)水平的影响。方法48只小鼠随机均分为对照组、ITP组和罗米司亭组。腹腔注射抗血小板抗体建立ITP模型,罗米司亭组皮下注射罗米司亭2 μg/kg。分别检测3组小鼠的血小板计数变化情况。使用实时荧光定量聚合酶链反应(qRT-PCR)、Western blot、流式细胞术检测BMNC中Notch和Jagged1,以及PBMC中GATA-3和PD-1水平。结果建模后小鼠血小板计数显著降低(P＜0.05),罗米司亭干预第7天和第14天罗米司亭组血小板计数显著高于ITP组(P＜0.05)。建模后小鼠BMNC中Notch信号通路表达水平显著升高(P＜0.05),罗米司亭组Notch、Jagged1蛋白和mRNA表达水平显著低于ITP组(P＜0.05)。建模后小鼠GATA-3 mRNA和PD-1水平显著降低(P＜0.05),罗米司亭组GATA-3 mRAN和PD-1水平显著高于ITP组(P＜0.05)。结论罗米司亭可提高ITP小鼠血小板计数,并且抑制BMNC中Notch信号通路表达水平,上调PBMC中GATA-3和PD-1表达。 |
| 英文摘要: |
| To explore the effects of Romipristine on Notch signal in mononuclear cells (BMNC) in mice with thrombocytopenic purpura, transfer factor binding protein-3 (GATA-3) in bone marrow monouclear cells(PBMC) and serumprogrammed cell death receptor-1(PD-1) levels. MethodsForty-eight mice were randomly divided into 3 groups, 16 each, namely the control group, the model group and the study group. The immune thrombocytopenic purpura was established by intraperitoneal injection of anti-platelet antibody, and the study group was injected with 2 μg/kg of rilastast. The changes in platelet counts of the three groups of mice were examined separately. Notch signaling, GTA-3 of PBMC and serum PD-1 levels in BMNC were detected by real time fluorescence quantitative polymerase chain reaction(qRT-PCR), Western blot or flow cytometry. ResultsThe platelet count of mice was significantly decreased after modeling (P＜0.05). The platelet count of the study group was significantly higher than that of the model group at 7 days after intervention and 14 days after intervention (P＜0.05). The expression level of Notch signaling pathway in mouse BMNC was significantly up-regulated after modeling (P＜0.05). The expression levels of Notch, Jagged1 protein and mRNA in the study group were significantly lower than those in the model group (P＜0.05). The levels of GATA-3 and PD-1 in mice were significantly decreased after modeling (P＜0.05). The GATA-3mRAN and PD-1 levels of the study group were significantly higher than the model group (P＜0.05). ConclusionRomipristine can increase the platelet count of immune thrombocytopenic purpura mice, and inhibit the expression of Notch pathway in BMNC, and up-regulate the expression of GATA-3 and serum PD-1 in PBMC. |
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