| 江岚,邓强,龙鼎新.人PKCα蛋白结构和功能的生物信息学分析.[J].中南医学科学杂志.,2022,(1):41-45. |
| 人PKCα蛋白结构和功能的生物信息学分析 |
| Bioinformatics analysis of Structure and Function of Human PKCα Protein |
| 投稿时间：2021-03-18 修订日期：2021-06-27 |
| DOI：10.15972/j.cnki.43-1509/r.2022.01.009 |
| 中文关键词: PKCα 生物信息学 人 蛋白结构 功能 |
| 英文关键词:PKCα bioinformatics human protein structure function |
| 基金项目:国家自然科学基金(81673227；81172712)；湖南省教育厅科学研究项目(19C0660) 作者简介:江岚,硕士,讲师,研究方向为神经毒理学,E-mail为616185307@qq.com。通信作者龙鼎新,博士,教授,博士研究生导师,研究方向为神经毒理学,E-mail为dxlong99@163.com。 |
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| 中文摘要: |
| 目的进行人蛋白激酶Cα(PKCα)蛋白结构和功能的生物信息学分析,为人PKCα的调控机制及生物学功能提供理论基础。方法利用NCBI、TMHMM、ProtComp、ProtParam等数据库和生物信息学分析工具,对人PKCα蛋白的结构、性质、功能域及蛋白质空间结构进行分析。结果人PKCα属不稳定蛋白,为非跨膜亲水性蛋白,无信号肽属于非分泌性蛋白,具有磷酸化位点。其二级结构主要是无规则卷曲,三级结构主要是蛋白质扭曲。无二硫键,有离子通道活性和钙释放通道活性,参与离子转运并与多种蛋白存在相互作用关系。结论人PKCα蛋白在参与和调节离子转运、细胞死亡、能量代谢等许多生理过程中起着重要的作用。 |
| 英文摘要: |
| To contribute theoretically to further research on regulatory mechanisms and biological functions of protein kinase C alpha(PKCα). MethodsModern bioinformatics online databases and analytic tools, including NCBI, Protscale, TMHMM, ProtComp and ProtParam, were used to analyze the possible structure and potential functions of human PKCα protein. ResultsThe human PKCα was an unstable protein, non-transmembrane protein and hydrophilic protein. PKCα had no signal peptide and belonged to non-secretory protein. The secondary structure of human PKC was mainly irregular curly. Moreover, the protein had multiple phosphorylation sites, but had no disulfide bonds. They had ion channel activity and calcium release channel activity, participated in ion transport and interacted with various proteins. ConclusionHuman PKCα play an important role in participating in ion transport, cell death, energy metabolism and many other physiological processes. |
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