谭武鹏,谭雄,伍菊花.二氢杨梅素通过PI3K/Akt信号通路调节巨噬细胞极化抑制卵巢癌细胞的侵袭.[J].中南医学科学杂志.,2021,(6):636-639.
二氢杨梅素通过PI3K/Akt信号通路调节巨噬细胞极化抑制卵巢癌细胞的侵袭
Dihydromyricetin inhibits invasion of ovarian cancer cells by regulating macrophage polarization through PI3K/Akt pathway
投稿时间:2020-12-07  修订日期:2021-09-07
DOI:10.15972/j.cnki.43-1509/r.2021.06.004
中文关键词:  二氢杨梅素  PI3K/Akt信号通路  巨噬细胞极化  卵巢癌  侵袭
英文关键词:dihydromyricetin  PI3K/Akt signaling pathway  macrophage polarization  ovarian cancer  invasion
基金项目:衡阳市科技计划项目(S2018N9031034363) 作者简介:谭武鹏,硕士,主治医师,研究方向为妇科肿瘤的防治,E-mail为tanwupeng@126.com。
作者单位E-mail
谭武鹏 衡阳市妇幼保健医院妇科,湖南省衡阳市421001 e-mail为tanwupeng@126.com 
谭雄 衡阳市妇幼保健医院妇科,湖南省衡阳市421001  
伍菊花 衡阳市妇幼保健医院妇科,湖南省衡阳市421001  
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中文摘要:
      目的观察二氢杨梅素(DMY)处理的RAW264.7巨噬细胞对人卵巢癌SKOV3细胞增殖和侵袭的影响。方法用DMY(60 mg/L)处理RAW细胞24 h,再将SKOV3和RAW细胞非接触共培养24 h。细胞计数试剂盒-8(CCK-8)和Transwell检测SKOV3细胞增殖和侵袭能力。酶联免疫吸附试验(ELISA)检测培养液上清中细胞因子水平。Western blot检测磷脂酰肌醇3-激酶(PI3K)、p-PI3K、丝氨酸-苏氨酸激酶(Akt)和p-Akt的表达。结果与SKOV3组比较,RAW+SKOV3组细胞增殖和侵袭细胞数显著升高(P<0.05)。与RAW+SKOV3组比较,DMY+RAW+SKOV3组细胞增殖和侵袭细胞数显著减少(P<0.05)。与RAW组比较,DMY+RAW组干扰素-γ、肿瘤坏死因子-α水平、PI3K、p-PI3K、Akt和p-Akt蛋白表达均显著升高,而白细胞介素-10和转化生长因子-β水平显著降低(P<0.05)。结论DMY抑制巨噬细胞诱导的SKOV3细胞增殖和侵袭,其机制可能与通过PI3K/Akt通路调节巨噬细胞极化有关。
英文摘要:
      To observe the effects of dihydromyricetin (DMY) on the proliferation and invasion of human ovarian cancer cell line SKOV3 cells. MethodsRaw cells were treated with DMY (60 mg / L) for 24 h, and then SKOV3 and RAW cells were co-cultured for 24 h. Cell counting kit-8 (CCK-8) and Transwell were used to detect the proliferation and invasion of SKOV3 cells. The concentration of cytokines in the supernatant of culture medium was detected by enzyme-linked immunosorbent assay (ELISA). Western blot was used to detect the expressions of phosphatidylinositol 3-kinase (PI3K), p-PI3K, serine threonine kinase (Akt) and p-Akt. ResultsThe proliferation level and the number of invasive cells in RAW+SKOV3 group were significantly increased compared with SKOV3 group (P<0.05). Compared with RAW+SKOV3 group, the proliferation level and the number of invasive cells in DMY+RAW+SKOV3 group were significantly decreased (P<0.05). Compared with RAW group, the levels of interferon gamma-γ and tumor necrosis factor-α, the protein expression levels of PI3K, p-PI3K, Akt and p-Akt in DMY+RAW group were significantly increased, while the levels of IL-10 and TGF-β were significantly decreased (P<0.05). ConclusionDMY inhibits the proliferation and invasion of SKOV3 cells induced by macrophages, and its mechanism may be related to the regulation of macrophage polarization through PI3K/Akt pathway.
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