| 肖萍,李通,杨晓玉,李美香.间质蛋白Periostin高表达对鼻咽癌6-10B细胞凋亡的影响及可能机制.[J].中南医学科学杂志.,2021,(6):621-625. |
| 间质蛋白Periostin高表达对鼻咽癌6-10B细胞凋亡的影响及可能机制 |
| Effect of high expression of Periostin on nasopharyngeal carcinoma 6-10B cell apoptosis and its possible mechanism |
| 投稿时间：2021-06-24 修订日期：2021-08-07 |
| DOI：10.15972/j.cnki.43-1509/r.2021.06.001 |
| 中文关键词: 鼻咽癌 细胞凋亡 Periostin P53 |
| 英文关键词:nasopharyngeal carcinoma apoptosis Periostin P53 |
| 基金项目:国家自然科学基金资助项目(81072199);衡阳市科技厅项目(2017KJ304) 作者简介:肖萍,初级实验师,研究方向为肿瘤发生机理,E-mail为799180803@qq.com。通信作者李美香,博士,硕士研究生导师,教授,研究方向为肿瘤发生机理,E-mail为meixiangle@163.com。通信作者杨晓玉,硕士,主管技师,研究方向为临床血液检测和分子检测,E-mail为296044244@qq.com。 |
|
| 摘要点击次数: 337 |
| 全文下载次数: 162 |
| 中文摘要: |
| 目的探讨间质蛋白Periostin高表达对鼻咽癌6-10B细胞凋亡的影响及其可能机制。方法流式细胞术及Western blot技术检测Periostin高表达前后6-10B细胞凋亡率的变化及凋亡相关蛋白Bax、Bcl-2、P53和p-Akt的表达；用稳定转染POSTN的6-10B细胞(6-10B POSTN细胞)及对照组6-10B系列细胞的培养上清,分别刺激6-10B细胞,检测刺激前后6-10B细胞中凋亡相关蛋白Bax、Bcl-2、P53和p-Akt的表达。将人P53 shRNA干扰载体转染到6-10B、6-10B-GFP和6-10B-POSTN细胞中,流式细胞术检测干扰P53后细胞的凋亡率。结果与对照组比较,Periostin高表达后6-10B细胞凋亡率明显升高,Bax和P53蛋白表达上调,Bcl-2的表达差异无显著性,但Bax/Bcl-2比值显著升高,p-Akt蛋白表达显著下调。Periostin高表达的6-10B细胞培养上清培养6-10B后,凋亡率较对照组显著升高。下调6-10B细胞中P53基因后,6-10B细胞的凋亡率较对照组无明显改变,而Periostin高表达的6-10B细胞的凋亡率较对照组显著升高。结论Periostin蛋白的表达上调可以促进鼻咽癌细胞的凋亡；其促凋亡机制与鼻咽癌组织中P53蛋白表达上调无关。 |
| 英文摘要: |
| To investigate the effect and mechanism of Periostin on 6-10B cells of nasopharyngeal carcinoma. MethodsThe apoptosis rate of 6-10B cells stably transfected with Periostin was detected by flow cytometry. The expression of apoptosis related proteins Bax, Bcl-2, P53 and p-Akt in 6-10B cells was detected by Western blot. The culture supernatants of 6-10B series cells stably transfected with Periostin and the control group were used to stimulate 6-10B cells respectively. The expressions of apoptosis related proteins Bax, Bcl-2, P53 and p-Akt in 6-10B cells before and after stimulation were detected. Human P53 shRNA interference vector was transfected into 6-10B, 6-10B-GFP and 6-10B-POSTN cells. The apoptosis rate was detected by flow cytometry. ResultsCompared with the control group, the apoptosis rate of 6-10B cells increased significantly, the expression of Bax and P53 protein increased, and the expression of Bcl-2 had no significant difference, but the ratio of Bax / Bcl-2 increased significantly and the expression of p-Akt protein decreased significantly. The apoptosis rate of 6-10B cells with high Periostin expression was significantly higher than that of the control group. After downregulating P53 gene in 6-10B cells, the apoptosis rate of 6-10B cells was not significantly changed compared with the control group, but the apoptosis rate of 6-10B cells transfected with Periostin was significantly higher than the control group. ConclusionsPeriostin upregulation can promote the apoptosis of nasopharyngeal carcinoma cells. The mechanism of promoting apoptosis is independent with the increased expression of P53 protein in nasopharyngeal carcinoma. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|