| 何宝凤,雷俊悦,曾奎,陈雄英,黄秋林.Apelin-13对肝细胞癌HepG2细胞增殖的影响.[J].中南医学科学杂志.,2021,(4):389-392. |
| Apelin-13对肝细胞癌HepG2细胞增殖的影响 |
| The effect of Apelin-13 on the proliferation of HepG2 cells |
| 投稿时间：2020-09-16 修订日期：2021-03-15 |
| DOI：10.15972/j.cnki.43-1509/r.2021.04.004 |
| 中文关键词: Apelin-13 肝细胞癌 增殖 凋亡 |
| 英文关键词:Apelin-13 hepatocellular carcinoma proliferation apoptosis |
| 基金项目:湖南省自然科学基金省市联合基金(2020JJ6055) |
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| 中文摘要: |
| 目的探讨Apelin-13对肝细胞癌HepG2细胞增殖的影响。方法将HepG2细胞按Apelin-13不同浓度分组:10%胎牛血清组(对照组)、0.000 1、0.001、0.01、0.1 μmol/L Apelin-13组,处理时间为24 h；按Apelin-13时效关系分组:0、6、12、18、24 h组,处理浓度为0.1 μmol/L。细胞增殖实验检测各组处理后细胞水平。蛋白质印迹法检测细胞增殖相关蛋白细胞周期蛋白D1(CyclinD1)和细胞凋亡相关蛋白半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的表达水平。结果HepG2细胞增殖程度随Apelin-13浓度增加及作用时间延长而增强(P＜0.05)。CyclinD1蛋白表达水平随Apelin-13处理浓度的升高及处理时间延长而升高(P＜0.05)；Caspase-3蛋白表达水平随Apelin-13处理浓度的升高及处理时间延长而降低(P＜0.05)。结论Apelin-13在一定范围内促进肝细胞癌HepG2细胞增殖,抑制Caspase-3蛋白的表达,抑制效果随着处理时间延长和浓度的升高而增强。 |
| 英文摘要: |
| To investigate the effect of Apelin-13 on proliferation of HepG2 cells. Methods HepG2 cells were divided into different concentrations of Apelin-13:10%FBS (control), 0.000 1,0.001,0.01,0.1 μmol/L.And the different durations were 0 h (control), 6 h, 12 h, 18 h, 24 h. The number of cells in each group was calculated by MTT method. Western blot was used to detect the expressions of cell cycle protein D1 (CyclinD1) and cysteine aspartate protease 3 (Caspase-3). ResultsThe proliferation of HepG2 cells was increased with the increase of Apelin-13 concentration, and the prolongation of the Apelin-13 treatment time (P＜0.05). The expression of CyclinD1 was increased with the increase of Apelin-13 concentration and treatment time, while the expression of Caspase-3 decreased with the increase of Apelin-13 concentration and treatment time. ConclusionApelin-13 promoted the proliferation of hepatocellular carcinoma HepG2 cells and inhibited the expression of Caspase-3 protein within a certain range, and the inhibitory effect was enhanced with prolongation of treatment time and the increase of treatment concentration. |
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