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邓云丽,刘海灿,屈勇刚,陈紫昕,宋德强,梁晏,张家瑞,万康林,刘志广,袁秀琴.基于宏基因组学的新疆牧场牛潜在人兽共患病原菌调查.[J].中南医学科学杂志.,2021,(3):253-258.

基于宏基因组学的新疆牧场牛潜在人兽共患病原菌调查

Investigation of potential zoonotic pathogens in Xinjiang based on metagenomics

投稿时间：2021-01-04

DOI：10.15972/j.cnki.43-1509/r.2021.03.002

英文关键词:cattle herds bacterial diversity metagenomic next-generation sequencing zoonosis

基金项目:

作者	单位	E-mail
邓云丽	南华大学公共卫生学院,湖南省衡阳市421001 中国疾病预防控制中心传染病预防控制所国家重点实验室, 北京市102206	e-mail为1016827326@qq.com,e-mail为wtjysh@126.com,e-mail为liuzhiguang@icdc.cn
刘海灿	中国疾病预防控制中心传染病预防控制所国家重点实验室, 北京市102206
屈勇刚	石河子大学动物科技学院,新疆维吾尔自治区石河子市832003
陈紫昕	南华大学公共卫生学院,湖南省衡阳市421001 中国疾病预防控制中心传染病预防控制所国家重点实验室, 北京市102206
宋德强	新疆生产建设兵团第八师石河子市动物卫生监督所,新疆维吾尔自治区石河子市832000
梁晏	石河子大学动物科技学院,新疆维吾尔自治区石河子市832003
张家瑞	石河子大学动物科技学院,新疆维吾尔自治区石河子市832003
万康林	中国疾病预防控制中心传染病预防控制所国家重点实验室, 北京市102206
刘志广	中国疾病预防控制中心传染病预防控制所国家重点实验室, 北京市102206	e-mail为1016827326@qq.com,e-mail为wtjysh@126.com,e-mail为liuzhiguang@icdc.cn
袁秀琴	南华大学公共卫生学院,湖南省衡阳市421001	e-mail为1016827326@qq.com,e-mail为wtjysh@126.com,e-mail为liuzhiguang@icdc.cn

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中文摘要:

目的调查新疆牧场规模化养殖牛携带的细菌多样性以及潜在细菌性人兽共患病致病菌种类,为牛规模化养殖过程中人兽共患病的防治提供科学依据。方法选取新疆地区规模化养殖牧场牛群,同时采集牛鼻咽拭子和牛肛拭子样品,提取全基因组DNA进行宏基因组分析。采用宏基因组二代测序,采用比对Minikrakenv2数据库及生物信息学方法,对该地区牛携带的微生物菌群及其潜在病原菌种类进行分析。结果宏基因组检测结果显示,所有牛样品中微生物菌群均以细菌为主。不同类别牛样品比较发现,牛肛拭子样品和牛鼻咽拭子样品均以厚壁菌门、放线菌门、变形菌门和拟杆菌门占主导地位,但两种类别牛样品的优势菌属各不相同,两者共有的优势菌属为异壁放线菌菌属和芽孢杆菌属。样品中共发现蜡样芽孢杆菌、大肠杆菌、猪链球菌、肺炎链球菌、鲍曼不动杆菌、肺炎克雷伯氏菌、空肠弯曲杆菌、化脓性链球菌和多杀巴斯德杆菌等9种人兽共患病原菌,其中大部分都是食源性细菌性致病菌。PCoA和ANOSIM生物信息学分析结果表明,两组样品、两个地区牛携带的细菌群落多样性以及潜在的人兽共患病原菌之间的差异均具有统计学意义(P＜0.05)。结论新疆地区规模化养殖牧场牛鼻咽拭子和牛肛拭子样品携带的细菌结构均呈现较丰富的多样性,并检出了以食源性传播为主的人兽共患病原菌,存在一定的食品安全和疾病传播隐患。

英文摘要:

To investigate bacterial diversity and potential bacterial zoonotic pathogens carried by cattles in large-scale farming ranches in Xinjiang, China, to provide a scientific basis for zoonosis control in the process of large-scale cattle breeding. MethodsBoth nasopharyngeal swab and anal swab samples were collected from the cattles of large-scale breeding farms selected in Xinjiang Uygur Autonomous Region region, China. The genomic DNA in the samples was extracted for microbial macrogenomic analysis. The microbial flora carried by the cattles in the region and the species of potential pathogens were analyzed by means of macrogenomic second-generation sequencing, comparing the Minikrakenv2 database and bioinformatics methods. ResultsThe results of macrogenomic analysis showed that the microflora of all bovine samples were dominated by bacteria. Comparative analysis of different sample categories revealed that Phylum Firmicutes, Phylum Actinobactereia, Phylum Proteobacteria and Phylum Bacteroidetes were dominated in both bovine anal swabs and bovine nasopharyngeal swabs samples, while the dominant bacteria genera in two sample categories were different, and the common dominant bacteria genera of the two samples were Actinobactereia and Bacillus. A total of nine zoonotic pathogens were found, including Bacillus cereus, Escherichia coli, Streptococcus suis, Streptococcus pneumoniae, Acinetobacter baumannii, Klebsiella pneumoniae, Campylobacter jejuni, Streptococcus pyogenes and Pasteurium multocidum, most of which were food-borne bacterial pathogens. The results of PCoA and ANOSIM analysis showed that there were statistically significant differences in bacterial community diversity and potential zoonotic pathogens between two groups of samples, cattles from two regions in Xinjiang(P＜0.05). ConclusionThe bacterial structures carried by bovine nasopharyngeal swabs and anal swab samples from large-scale breeding pastures in Xinjiang showed rich diversity and zoonotic pathogens mainly transmitted by foodborne pathogens were detected, which presented a certain risk of food safety and disease transmission.

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