| 曹晓丽,苏世文,贾英.LRP11在宫颈癌中表达及其与HPV感染和肿瘤进展的关系.[J].中南医学科学杂志.,2021,(1):25-30. |
| LRP11在宫颈癌中表达及其与HPV感染和肿瘤进展的关系 |
| Expression of LRP11 in cervical cancer tissues and its relationship with HPV infection and tumor progression |
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| DOI：10.15972/j.cnki.43-1509/r.2021.01.005 |
| 中文关键词: LRP11 宫颈癌 HPV感染 细胞增殖 细胞侵袭 细胞凋亡 |
| 英文关键词:LRP11 cervical cancer HPV infection cell proliferation cell invasion apoptosis |
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| 中文摘要: |
| 目的研究低密度脂蛋白受体相关蛋白11(LRP11)在宫颈癌中表达及其与人乳头瘤病毒(HPV)感染和肿瘤进展的关系。方法收集123例宫颈癌手术患者的癌组织(HPV阴性25例,HPV阳性98例)及癌旁正常组织,采用免疫组织化学染色法检测癌旁正常组织、HPV阴性宫颈癌组织、HPV阳性宫颈癌组织中LRP11蛋白表达水平。选用HPV阳性人宫颈癌细胞系Hela、HPV阴性人宫颈癌细胞系C33A及人正常宫颈上皮细胞系HUCECs,采用Western blot法检测3种细胞中LRP11蛋白表达量。将Hela细胞分为对照组、空质粒组和LRP11质粒组,对照组不作任何处理,空质粒组转染8 μg pEGFP-N1质粒,LRP11质粒组转染8 μg pEGFP-N1-LRP11质粒,CCK8法检测各组Hela细胞增殖率,Transwell小室侵袭实验检测各组Hela细胞侵袭能力,流式细胞术检测各组Hela细胞凋亡率,Western blot法检测各组Hela细胞中LRP11蛋白表达量。结果HPV阴性宫颈癌组织和HPV阳性宫颈癌组织中LRP11阳性表达率高于癌旁正常组织(P＜0.05),HPV阳性宫颈癌组织LRP11阳性表达率高于HPV阴性宫颈癌组织(P＜0.05)。Hela细胞和C33A细胞LRP11蛋白表达量高于HUCECs细胞(P＜0.05),Hela细胞LRP11蛋白表达量高于C33A细胞(P＜0.05)。LRP11质粒组Hela细胞增殖率、穿过小室膜的Hela细胞数、LRP11蛋白表达量较对照组和空质粒组高(P＜0.05),LRP11质粒组Hela细胞凋亡率较对照组和空质粒组低(P＜0.05),空质粒组Hela细胞增殖率、凋亡率、穿过小室膜的Hela细胞数、LRP11蛋白表达量较对照组差异无统计学意义(P＞0.05)。结论LRP11在宫颈癌中呈高表达,宫颈癌中LRP11表达与HPV感染有一定相关性；LRP11过表达可促进宫颈癌细胞增殖和侵袭,抑制其凋亡,以加速肿瘤进展。 |
| 英文摘要: |
| To investigate the expression of low-density lipoprotein receptor-related protein 11 (LRP11) in cervical cancer tissues and its relationship with human papillomavirus (HPV) infection and tumor progression. MethodsThe cancer tissues and adjacent normal tissues of 123 cervical cancer patients (25 cases of HPV negative and 98 cases of HPV positive) in our hospital were collected. Immunohistochemical staining was used to detect the expression level of LRP11 protein in adjacent normal tissues, HPV-negative cervical cancer tissue, and HPV-positive cervical cancer tissue. The HPV-positive human cervical cancer cell line Hela, HPV-negative human cervical cancer cell line C33A, and human normal cervical epithelial cell line HUCECs were selected. Western blot method was used to detect the expression of LRP11 protein in the three cell lines. Hela cells were divided into control group, empty plasmid group, and LRP11 plasmid group. Control group was untreated, empty plasmid group was transfected with 8 μg pEGFP-N1 plasmid, and LRP11 plasmid group was transfected with 8 μg pEGFP-N1-LRP11 plasmid. The proliferation rate, invasion ability and apoptosis rate of Hela cells in each group were detected by CCK8 assay, Transwell chamber assay and flow cytometry, then the expression of LRP11 protein in Hela cells of each group was detected by Western blot. ResultsThe positive expression rate of LRP11 among three kinds of tissue was the highest in HPV-positive cervical cancer tissue, followed by HPV-negative cervical cancer tissue, and was the lowest in adjacent normal tissue, with statistic difference (P＜0.05). The LRP11 protein expression level among three cell lines was the highest in Hela cell line, followed by C33A cell line, and was the lowest in HUCECs cell line, with significant difference (P＜0.05). The proliferation rate, number of transmembrane cells, and LRP11 protein expression of Hela cells in LRP11 plasmid group were higher than those of control group and empty plasmid group (P＜0.05), and the apoptosis rate of Hela cells in LRP11 plasmid group was lower than that of control group and empty plasmid group (P＜0.05). The proliferation rate, apoptosis rate, number of transmembrane cells, and LRP11 protein expression showed no significant difference between control group and empty plasmid group (P＞0.05). ConclusionThe elevated expression of LRP11 in cervical cancer is related to HPV infection, indicating that the overexpression of LRP11 can promote the proliferation and invasion of cervical cancer cells, inhibit their apoptosis, and accelerate tumor progression. |
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