| 曾莎莎,肖玲巧,唐瑶,段洁,张洁雅,李蕊,郭开云,张艳.幽门螺杆菌Tipα通过Wnt/β-catenin通路诱导胃癌细胞EMT.[J].中南医学科学杂志.,2020,(6):618-623. |
| 幽门螺杆菌Tipα通过Wnt/β-catenin通路诱导胃癌细胞EMT |
| Tipα protein of Helicobacter pylori induces EMT in gastric cancer cell through Wnt/β-catenin signaling pathway |
| 投稿时间：2020-07-08 修订日期：2020-10-20 |
| DOI：10.15972/j.cnki.43-1509/r.2020.06.016 |
| 中文关键词: 幽门螺杆菌 肿瘤坏死因子α诱导蛋白(Tipα) Wnt/β-catenin信号通路 胃癌 上皮-间质转化(EMT) |
| 英文关键词:Helicobacter pylori TNF-α inducing protein (Tipα) Wnt/β-catenin pathway gastric cancer epithelial-mesenchymal transition(EMT) |
| 基金项目: |
| 作者 | 单位 | | 曾莎莎 | 南华大学 衡阳医学院病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南 衡阳 421001 | | 肖玲巧 | 南华大学 衡阳医学院病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南 衡阳 421001 | | 唐瑶 | 南华大学 衡阳医学院病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南 衡阳 421001 | | 段洁 | 南华大学 衡阳医学院病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南 衡阳 421001 | | 张洁雅 | 南华大学 衡阳医学院病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南 衡阳 421001 | | 李蕊 | 南华大学 护理学院,湖南 衡阳 421001 | | 郭开云 | 南华大学 衡阳医学院病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南 衡阳 421001 | | 张艳 | 南华大学 衡阳医学院病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南 衡阳 421001 |
|
| 摘要点击次数: 745 |
| 全文下载次数: 623 |
| 中文摘要: |
| 探讨幽门螺杆菌(H.pylori)肿瘤坏死因子α诱导蛋白(Tipα)是否通过Wnt/β联蛋白(β-catenin)信号通路诱导人胃癌SGC7901细胞上皮-间质转化(EMT)。设置PBS组、Tipα组和通路抑制剂XAV939预处理组,采用Western blot和免疫荧光观察β-catenin磷酸化和核转位情况；qRT-PCR和Western blot检测E-钙黏蛋白(E-cadherin)、紧密连接蛋白(ZO-1)、N-钙黏蛋白(N-cadherin)和波形蛋白(Vimentin)以及通路下游靶基因(c-myc和cyclinD1)的表达情况；划痕实验和Transwell实验检测细胞迁移能力。结果显示,Tipα能以时间依赖性方式诱导β-catenin Ser675和Ser552磷酸化和核转位,且下调E-cadherin和ZO-1表达,上调N-cadherin和Vimentin表达(P＜0.01)；与PBS相比,Tipα增强c-myc和cyclinD1的表达(P＜0.05)以及细胞的迁移能力(P＜0.05)。XAV939预处理抑制Tipα诱导的SGC7901细胞EMT形成及通路下游靶基因的表达。表明Wnt/β-catenin信号通路参与了Tipα诱导的胃癌细胞EMT形成。 |
| 英文摘要: |
| To investigate whether the tumor necrosis factor-α-inducing protein (Tipα) of Helicobacter pylori (H.pylori) induces epithelial-mesenchymal transformation (EMT) in human gastric cancer SGC7901 cell through the Wnt/β-catenin signaling pathway. The phosphorylation and nuclear translocation of β-catenin were observed by Western blot and immunofluorescence respectively in the PBS group, Tipα group and the pathway inhibitor XAV939 pretreatment group. qRT-PCR and Western blot were employed to detect the expression of E-cadherin, ZO-1, N-cadherin and Vimentin, as well as downstream target genes (c-myc and cyclinD1) of the Wnt/β-catenin signaling pathway. The cell migration ability was measured by wound healing assay and transwell assay. The results showed that Tipα induced β-catenin (Ser675 and Ser552) phosphorylation and nuclear translocation in SGC7901 cell in a time-dependent manner. Moreover, Tipα down-regulated the expression of E-cadherin and ZO-1, and up-regulated the expression of N-cadherin and Vimentin (P＜0.01). Compared with PBS treatment, Tipα increased the expression of c-myc and cyclinD1 (P＜0.05) and enhanced the migration ability of SGC7901 cell (wound healing, P＜0.05; Transwell, P＜0.01). However, XAV939 pretreatment inhibited Tipα-induced EMT and downstream target gene expression in SGC7901 cell. This suggested that Wnt/β-catenin signaling pathway was involved in Tipα-induced EMT in gastric cancer cell. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|