| 徐庆东,郭焕开,苏明,陈洁欣,马惠娟,邓继鸿,姜松青,石晓峰.糖尿病肾组织PPARγ和PTEN的表达及对EMT和纤维化的影响.[J].中南医学科学杂志.,2020,(5):499-504. |
| 糖尿病肾组织PPARγ和PTEN的表达及对EMT和纤维化的影响 |
| Effect of PPARγ and PTEN expression in diabetic renal tissue on EMT and fibrosis |
| 投稿时间：2019-06-19 修订日期：2020-06-08 |
| DOI：10.15972/j.cnki.43-1509/r.2020.05.014 |
| 中文关键词: 糖尿病肾病 肾小管上皮细胞 过氧化物酶体增殖物激活受体 PTEN 上皮细胞间质转化 纤维化 |
| 英文关键词:diabetic nephropathy renal tubular epithelial cells PPARγ PTEN epithelial-mesenchymal transition fibrosis |
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| 中文摘要: |
| 研究糖尿病小鼠肾组织及高糖培养的肾小管上皮细胞中过氧化物酶体增殖物激活受体γ(PPARγ)及第10号染色体缺失的磷酸酶和张力蛋白同源基因(PTEN)的表达情况及对上皮细胞间质转化(EMT)和纤维化情况。取糖尿病小鼠肾组织和高糖培养的肾小管上皮细胞,采用Western Blot检测蛋白表达；聚合酶链式反应(PCR)检测mRNA表达,分析PTEN与PPARγ、α-平滑肌肌动蛋白(α-SMA)、胶原蛋白Ⅲ(CollagenⅢ)、上皮钙粘蛋白(E-cadherin)的表达表达量的相关性。结果发现,糖尿病小鼠肾组织和高糖培养肾小管上皮细胞PPARγ、PTEN蛋白及mRNA表达均明显降低,E-cadherin蛋白表达下调,α-SMA、Collagen-Ⅲ蛋白表达上调,肾小管上皮细胞的EMT蛋白表达显著降低,肾组织纤维化现象明显加重。结果提示高糖条件下PPARγ、PTEN表达降低可能与EMT和纤维化过程有关。 |
| 英文摘要: |
| To investigate the expression of peroxisome proliferator-activated receptor γ (PPARγ) and phosphatase and tensin homologue deleted on chromosome ten (PTEN) in diabetic renal tissue and high glucose cultured renal tubular epithelial cells and its effect on epithelial-mesenchymal transition (EMT) and fibrosis. The renal tissues of diabetic mice and renal tubular epithelial cells cultured with high glucose were collected. Western Blot was used to detect the expressions of proteins, and polymerase chain reaction (PCR) was used to detect the mRNA expression. The correlation between PTEN and PPARγ, E-cadherin, α-SMA and Collagen-Ⅲ protein expression was analyzed. The results showed that the mRNA and protein expression levels of PPARγ and PTEN were down-regulated in both renal tissues of diabetic mice and renal tubular epithelial cells cultured with high glucose, and expression of E-cadherin protein was also down-regulated, but the α-SMA, and Collagen-Ⅲ protein expression were up-regulated, moreover, EMT and renal fibrosis of renal tubular epithelial cells were significantly aggravated. These results indicate that the expression levels of PPARγ and PTEN may be involved in the process of EMT and fibrosis. |
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