| 王欣月,孙文锦.4-PBA对大鼠肺动脉高压的作用及药理机制.[J].中南医学科学杂志.,2020,(4):346-348, 367. |
| 4-PBA对大鼠肺动脉高压的作用及药理机制 |
| The effect and pharmacological mechanism of 4-PBA on pulmonary hypertension in rats |
| 投稿时间：2019-11-18 修订日期：2020-05-26 |
| DOI：10.15972/j.cnki.43-1509/r.2020.04.003 |
| 中文关键词: 4-苯基丁酸 肺动脉高压 大鼠 |
| 英文关键词:4- phenyl butyric acid pulmonary hypertension rats |
| 基金项目: |
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| 中文摘要: |
| 为探讨4-苯基丁酸(4-PBA)对大鼠肺动脉高压的作用及机制,选取SD大鼠60只,将大鼠随机分为对照组、模型组、4-PBA组,对照组、模型组给予等量生理盐水,4-PBA组分别给予250 mg/kg或500 mg/kg 4-PBA,连续4周,观察各组大鼠血流动力学(RVSP、mPAP、PASP)、右心室重构指数(RVHI)及肺血管壁厚占血管外径的百分比(MVT%)指标,采用Western blot检测蛋白表达,结果显示,与对照组比较,模型组RVSP、mPAP、PASP、RVHI、MVT等均显著升高,α-SMA、Bcl-2和Bax等蛋白表达均显著增加,给予4-PBA干预后,上述指标和蛋白表达显著下降。结果得出4-PBA可改善肺动脉高压大鼠血流动力学、右室和肺血管重构,可能与其下调α-SMA、Bcl-2和Bax蛋白表达有关。 |
| 英文摘要: |
| To investigate the effect and mechanism of 4-phenylbutyric acid (4-PBA) on pulmonary hypertension in rats, 60 SD rats were randomly divided into control group, model group, 4-PBA group. The control and model groups were given the same amount of saline, 4-PBA group was given 250 mg/kg or 500 mg/kg 4-PBA respectively for 4 weeks, the statuses of hemodynamic (RVSP、mPAP、PASP), right ventricular remodeling index (RVHI) and the percentage of pulmonary vessel wall thickness to vessel diameter (MVT%) were observed in each group. Western blot was used to detect the expressions of protein in lung tissue. The results showed, compared to control group, the value of RVSP, mPAP, PASP, RVHI, MVT% were elevated, and the protein expressions of α-SMA, Bcl-2 and Bax were significantly increased in the model, which were reversed by 4-PBA. In conclusion, 4-PBA can improve hemodynamics, right ventricular and pulmonary vascular remodeling in rats with pulmonary hypertension, which may be related to the down-regulation of α-SMA, Bcl-2 and Bax protein expression. |
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