| 王刚,姚丽霞,杨涛,彭克楠,郭留雄,魏东,谷守义,刘俊江.前列腺癌中微小RNA-362-3p的表达及意义.[J].中南医学科学杂志.,2020,(3):279-283. |
| 前列腺癌中微小RNA-362-3p的表达及意义 |
| Expression and significance of microRNA-362-3p in prostate adenocarcinoma |
| 投稿时间：2019-12-26 修订日期：2020-03-15 |
| DOI：10.15972/j.cnki.43-1509/r.2020.03.014 |
| 中文关键词: 前列腺癌 miR-362-3p 粘附素 细胞外基质 预后 |
| 英文关键词:prostate adenocarcinoma miR-362-3p adhesin extracellular matrix prognosis |
| 基金项目: |
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| 中文摘要: |
| 为探索前列腺癌中微小RNA-362-3p(miR-362-3p)与转移的关系,以前列腺癌组织作为观察组,前列腺增生组织作为对照组,每组67例。采用实时荧光定量PCR法检测miR-362-3p的表达,免疫组化法检测上皮型粘附素(E-cadherin)和基质金属蛋白酶-11(MMP-11)的表达,Western Blot法检测基质金属蛋白酶-14(MMP-14)的表达。结果显示:miR-362-3p在前列腺癌组织中低表达。miR-362-3p在前列腺癌患者的脉管癌栓、Gleason分级、转移和不同TNM分期的表达存在显著差别。miR-362-3p与前列腺癌患者的生存时间显著相关,miR-362-3p分别与MMP-11、MMP-14具有显著负相关性,但与E-cadherin具有显著正相关性。结果表明,miR-362-3p可能通过调节细胞粘附和细胞外基质参与前列腺癌转移,可能作为患者的预后标志物。 |
| 英文摘要: |
| To explore the relationship between microRNA-362-3p (miR-362-3p) and metastasis in prostatic adenocarcinoma, prostatic adenocarcinoma were selected as observation group, and benign prostatic hyperplasia as control group (n＝67). MiR-362-3p was detected by real-time fluorescence quantitative PCR (qPCR). Expressions of E-cadherin and MMP-11 were detected by immunohistochemistry method, MMP-14 was detected by Western Bloting. Result showed lower expression of miR-362-3p in the prostate adenocarcinoma, expression of miR-362-3p was significantly different in vascular tumor thrombus, gleason classification, metastasis and TNM stages. MiR-362-3p was correlated with survival time. Negative correlation was found between miR-362-3p and MMP-11 or MMP-14. Positive correlation was found between miR-362-3p and E-cadherin. The results suggested miR-362-3p may be involved in progression of prostate adenocarcinoma by regulating cell adhesion and extracellular matrix, and may be a prognostic marker. |
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