| 汤敏,唐建,罗坤,吴海峰,朱理,彭旭辉,汪翼,罗红梅.膀胱灌注青藤碱对MNU诱发雄性SD大鼠膀胱癌的抑制作用.[J].中南医学科学杂志.,2020,(2):191-195. |
| 膀胱灌注青藤碱对MNU诱发雄性SD大鼠膀胱癌的抑制作用 |
| Intervention effect of intravesical infusion of sinomenine on tumor immunity induced by MNU in male SD rats with bladder cancer |
| 投稿时间：2019-07-17 修订日期：2019-12-28 |
| DOI：10.15972/j.cnki.43-1509/r.2020.02.020 |
| 中文关键词: MNU 青藤碱 膀胱癌 肿瘤免疫 |
| 英文关键词:MNU sinomenine bladder cancer tumor immunity |
| 基金项目: |
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| 中文摘要: |
| 研究膀胱灌注青藤碱对N-甲基亚硝基脲(MNU)诱发大鼠膀胱癌的肿瘤免疫及细胞凋亡的影响。将SD大鼠随机分为对照组、模型组、青藤碱组、青藤碱+1/2BCG组、卡介苗(BCG)组等5组,建立大鼠膀胱癌模型,检测大鼠CTLA-4、PD-1、B7-H3的水平,评估大鼠膀胱病理结果。结果提示青藤碱组、青藤碱+1/2BCG组CTLA-4、PD-1均显著低于模型组,青藤碱+1/2BCG组、BCG组相比差异无显著性；膀胱炎症及HE染色评分均显著低于模型组。结果表明青藤碱能显著抑制大鼠膀胱癌CTLA-4、PD-1的表达,降低膀胱炎症及HE染色评分,抑制膀胱癌进展。 |
| 英文摘要: |
| To study the effects of bladder perfusion of sinomenine on tumor immunity and apoptosis of bladder cancer induced by N-methylnitrosourea (MNU) in rats. In the course of study, SD rats were randomly divided into five groups:control group, model group, sinomenine group, sinomenine+ half dose BCG group and BCG group. The purpose of this study was to evaluate the pathological results of rat bladder by establishing a rat Bladder cancer model to detect the levels of CTLA-4, PD-1, and B7-H3 in rats. The results showed that the scores of CTLA-4 and PD-1 were significantly lower than those in model group,and there was no significant difference between sinomenine+ half dose BCG and BCG group;cystitis and HE staining in sinomenine group and sinomenine+ half dose BCG group were significantly lower than those in model group. In summary, it has been confirmed that sinomenine can significantly inhibit the expression of bladder cancer CTLA-4 and PD-1 in rats, thus reducing the score of cystitis and HE staining, and finally inhibiting the progress of bladder cancer. |
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