| 汤中,蒋亦龙,彭辉灿.原花青素对高糖环境下人视网膜内皮细胞增殖及VEGF表达的影响.[J].中南医学科学杂志.,2020,(2):155-159. |
| 原花青素对高糖环境下人视网膜内皮细胞增殖及VEGF表达的影响 |
| Effects of proanthocyanidin on human retinal capillary endothelial cells proliferation and VEGF expression in high glucose environment |
| 投稿时间：2019-08-29 修订日期：2019-12-30 |
| DOI：10.15972/j.cnki.43-1509/r.2020.02.011 |
| 中文关键词: 原花青素 糖尿病视网膜病变 人视网膜血管内皮细胞 VEGF |
| 英文关键词:procyanidin diabetic retinopathy human retinal vascular endothelial cells VEGF |
| 基金项目: |
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| 中文摘要: |
| 探索原花青素(PC)对体外高糖环境下培养的人视网膜血管内皮细胞(HRCECs)增殖及其VEGF表达的影响。利用MTT比色法检测HRCECs增殖；蛋白质免疫印迹法测定VEGF表达。结果显示:与低糖组比较,高糖组HRCECs数量明显增多,表达量升高；不同浓度原花青素(200,400,600,800 μg/mL)可抑制高糖组HRCECs的增殖,高糖组VEGF表达并存在浓度和时间依赖性。与低糖组比较,高糖组HRCECs VEGF。结果表明,原花青素可抑制高糖诱导的体外培养的HRCECs增殖。 |
| 英文摘要: |
| To explore the effect of proanthocyanidins on proliferation and VEGF expression of human retinal vascular endothelial cells cultured in high glucose environment. The proliferation of HRCECs was detected by MTT colorimetric assay and the expression of VEGF was detected by immunoblotting. The results show that compared with the low glucose group, the number of HRCECs in the high glucose group was significantly increased. Different concentrations of proanthocyanidins (200,400,600,800 μg/mL) could inhibit the proliferation of HRCECs and the expression of VEGF in the high glucose group in a concentration and time dependent manner. The results showed that proanthocyanidins could inhibit the proliferation of HRCECs induced by high glucose in vitro. |
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