龙晓兰,张万勇,吴勇军,刘德勇.Cofilin-1与肝癌细胞迁移侵袭能力的相关性研究.[J].中南医学科学杂志.,2019,(6):571-575, 600.
Cofilin-1与肝癌细胞迁移侵袭能力的相关性研究
The correlation research of relationship between Cofilin-1 and the migration or invasion ability of hepatocellular carcinoma cells
投稿时间:2018-12-06  修订日期:2019-05-20
DOI:10.15972/j.cnki.43-1509/r.2019.06.003
中文关键词:  Cofilin-1  肝癌  迁移  侵袭
英文关键词:Cofilin-1  hepatocellular carcinoma  migration  invasion
基金项目:
作者单位
龙晓兰 湘潭市第一人民医院病理科,湖南 湘潭 411010 
张万勇 湘潭市第一人民医院病理科,湖南 湘潭 411010 
吴勇军 湘潭市第一人民医院病理科,湖南 湘潭 411010 
刘德勇 湖南环境生物职业技术学院,湖南 衡阳 421001 
摘要点击次数: 139
全文下载次数: 166
中文摘要:
      探讨丝切蛋白1(Cofilin-1)与肝癌细胞的迁移、侵袭能力的关系,运用免疫印迹试验(western blot)检测肝癌细胞(Huh-7细胞)、转染上皮生长因子受体的人肝癌细胞(Huh-7-EGFR细胞)与转染上皮生长因子受体突变体Ⅲ的人肝癌细胞(Huh-7-EGFRvⅢ细胞)三种细胞中Cofilin-1的蛋白表达。然后将Cofilin-1用慢病毒表达系统导入Huh-7细胞,构建转染丝切蛋白1的人肝癌细胞(Huh-7-Cofilin-1细胞)系,并应用迁移侵袭实验检测细胞迁移侵袭的能力;再用RNA干扰(siRNA)技术沉默Huh-7-EGFRvⅢ细胞中的Cofilin-1,然后应用迁移侵袭实验检测细胞的迁移侵袭能力。结果显示:转染Cofilin-1后,Huh-7细胞的迁移侵袭能力显著增强(P<0.05);而干扰Cofilin-1后,Huh-7-EGFRvⅢ细胞迁移与侵袭能力明显减弱(P<0.05)。表明 Cofilin-1参与肝癌细胞迁移与侵袭。
英文摘要:
      In order to study the relationship between cofilin-1 and the migration and invasion ability of hepatocellular carcinoma cells.The protein expression of Cofilin-1 in Huh-7 cells and Huh-7-EGFR cells and Huh-7-EGFRvⅢ cells were detected by western blot. Cofilin-1 was transfected into Huh-7 cells by lentivirus expression system. Cofilin-1 was silenced in the Huh-7-EGFRvⅢ cells.The migration or invasion ability of cells were analyzed and verified by transwell migration assay and matrigel invasion assay.The protein expression of Cofilin-1 in Huh-7-EGFRvⅢ cells was significantly higher than that in Huh-7 and Huh-7-EGFR cells. After transfection of Cofilin-1, the migration and invasion ability of Huh-7 cells were enhanced. Conversely,after interference Cofilin-1, the migration and invasion ability of Huh-7-EGFRvⅢ cells were decreased.In conclusion, the migration and invasion of Hepatocellular Carcinoma cells were promoted by Cofilin-1.
查看全文  查看/发表评论  下载PDF阅读器
关闭
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=3E82CCEFC220CC9159A1A7E127905FAED49C77FF673CB42552891A9ABF594E15EFEB8842923989032448446BCCD77C3C36D7DC528AFDA8F1143A5A8F277824E71F5BFC07B8B42D1F877F43B57AC3D47EC14E81C93B4C1AAE8127C4593995FB92E87F8139F16CDCC99E47CD9B2790FA7925C6B181D7AB201AEBF8C2B12EE5C1D800413D9E50C08302&pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=BB33F1C95224820A&jid=6A20DF2A798996E24F064D5ECF83A153&yid=B6351343F4791CA3&aid=7BA62EAAEF4CF3CAD973E385C74DDA29&vid=&iid=B31275AF3241DB2D&sid=F16C0F639D87527E&eid=84981563331867A8A1E47C60CF6D72DE&fileno=20190603&flag=1&is_more=0">