| 黄红丽,杨一,王桂芳,黄桓.CYTOR通过调控miR-125b-5p/HK2通路促进宫颈癌细胞有氧糖酵解、增殖和侵袭.[J].中南医学科学杂志.,2019,(4):367-373. |
| CYTOR通过调控miR-125b-5p/HK2通路促进宫颈癌细胞有氧糖酵解、增殖和侵袭 |
| CYTOR promotes aerobic glycolysis, proliferation and invasion of cervical cancer cells by regulating miR-125b-5p/HK2 pathway |
| 投稿时间：2019-05-06 修订日期：2019-05-23 |
| DOI：10.15972/j.cnki.43-1509/r.2019.04.008 |
| 中文关键词: 宫颈癌 增殖 侵袭 细胞骨架RNA 有氧糖酵解 |
| 英文关键词:cervical cancer proliferation invasion CYTOR aerobic glycolysis |
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| 中文摘要: |
| 探讨细胞骨架RNA(CYTOR)及其下游分子轴对宫颈癌细胞增殖和侵袭的影响。选取宫颈癌组织及细胞,检测CYTOR的表达水平；采用CCK-8、Transwell、有氧糖酵解方法检测探讨CYTOR对宫颈癌细胞增殖、侵袭和有氧糖酵解的影响；同时利用双荧光素酶报告基因验证CYTOR、小分子RNA miR-125b-5p、己糖激酶2(HK2)之间的相互作用关系。结果显示,CYTOR在宫颈癌组织及细胞中明显上调(P<0.05)；抑制CYTOR可明显降低宫颈癌细胞增殖、侵袭和有氧糖酵解(P<0.05)；荧光素酶报告基因证实CYTOR可竞争性吸附miR-125b-5p,而miR-125b-5p可负调控HK2的表达。研究表明,CYTOR/miR-125b-5p/HK2分子轴促进宫颈癌细胞增殖、侵袭和有氧糖酵解,是一个潜在的宫颈癌治疗分子靶点。 |
| 英文摘要: |
| To investigate the effect of lncRNA CYTOR/miR-125b-5p/HK2 axis on proliferation and invasion of cervical cancer cells, cervical cancer tissues and cells were selected to detect the expression level of CYTOR; after inhibiting CYTOR, CCK-8, Transwell, glycolysis assay was used to investigate the effects of CYTOR on proliferation, invasion and aerobic glycolysis of cervical cancer cells. The interaction between CYTOR/miR-125b-5/HK2 was verified by the dual luciferase reporter system. The results showed that CYTOR was significantly up-regulated in cervical cancer tissues and cells (P<0.05); inhibition of CYTOR significantly decreased proliferation, invasion and aerobic glycolysis of cervical cancer cells (P<0.05); luciferase reporter confirmed CYTOR competitively adsorbed miR-125b-5p, while miR-125b-5p negatively regulates the expression of HK2. The rescue experiment also confirmed that CYTOR increased the expression of HK2 through miR-125b-5p, which promoted the proliferation, invasion and aerobic glycolysis of cervical cancer cells (P<0.05). Our research indicates that the CYTOR/miR-125b-5p/HK2 axis promotes proliferation, invasion and aerobic glycolysis of cervical cancer cells and is a potential molecular target for cervical cancer therapy. |
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