| 彭芳,陈艳宇,王贵明,周扬帆.linc00152通过调控DNMT3A和DNMT3B促进人宫颈癌细胞生长与侵袭.[J].中南医学科学杂志.,2019,(4):358-362. |
| linc00152通过调控DNMT3A和DNMT3B促进人宫颈癌细胞生长与侵袭 |
| Linc00152 promotes the growth and invasion of human cervical cancer cells by regulating DNMT3A and DNMT3B |
| 投稿时间：2019-05-07 修订日期：2019-06-03 |
| DOI：10.15972/j.cnki.43-1509/r.2019.04.006 |
| 中文关键词: linc00152 宫颈癌 甲基化转移酶3A 甲基化转移酶3B 生长与侵袭 |
| 英文关键词:linc00152 cervical cancer methyltransferase 3A methyltransferase 3B growth and invasion |
| 基金项目: |
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| 中文摘要: |
| 探讨linc00152在宫颈癌中的生物学功能及其调控机制。运用qRT-PCR检测人宫颈癌细胞与正常宫颈上皮细胞中linc00152的表达；转染sh-linc00152、si-DNMT3A和si-DNMT3B于HeLa细胞以敲低细胞中linc00152、DNMT3A和DNMT3B的表达水平,采用qRT-PCR与Western blot法检测转染效率；采用Western blot法检测sh-linc00152对甲基化转移酶(DNMT)3A和3B蛋白表达水平的影响；采用MTT、Transwell侵袭实验检测sh-linc00152、si-DNMT3A以及si-DNMT3B对人宫颈癌HeLa细胞增殖与侵袭的影响。结果显示,linc00152在宫颈癌细胞中的表达水平明显高于正常宫颈上皮细胞(P<0.01)；在HeLa细胞中,sh-linc00152组DNMT3A和DNMT3B蛋白表达水平明显低于sh-control组；sh-linc00152组、si-DNMT3A以及si-DNMT3B组HeLa细胞的存活率及穿过基质胶的HeLa细胞数量均明显低于各自对照组。linc00152可能通过调控DNMT3A和DNMT3B信号通路促进人宫颈癌细胞生长与侵袭。 |
| 英文摘要: |
| To explore the biological function and regulation mechanism of linc00152 in cervical cancer. qRT-PCR was used to detect the expression of linc00152 in human cervical cancer cells and normal cervical epithelial cells. Transfection of sh-linc00152 and si-DNMT3A, as well as si-DNMT3B knockdown the expression of linc00152, DNMT3A, DNMT3B in HeLa cells, the efficiency of transfection were detected by qRT-PCR and Western blot. Western blot was conducted to detect the expressions of DNMT3A and DNMT3B protein regulated by sh-linc00152. The proliferation and invasion ability of human cervical cancer HeLa cells regulated by sh-linc00152 and si-DNMT3A, as well as si-DNMT3B in vitro were evaluated by MTT and transwell invasion assays. The expression of linc00152 in cervical cancer cells was significantly higher than that in normal cervical epithelial cells. Western blot showed that the level of DNMT3A and DNMT3B protein in sh-linc00152 group was significantly lower than that in sh-control group. The viability and the invasion ability of HeLa cells in sh-linc00152 group and si-DNMT3A, as well as si-DNMT3B group were significantly lower than those in the control group. linc00152 may promote the growth and invasion of human cervical cancer cells by regulating DNMT3A and DNMT3B signaling pathways. |
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