| 芮璐,刘锐,张本青,刘凯颺,沈刘忠,张晶,王旭,于存涛,王巍.MAD1基因在主动脉夹层中的表达及对主动脉平滑肌细胞的影响.[J].中南医学科学杂志.,2019,(3):245-249. |
| MAD1基因在主动脉夹层中的表达及对主动脉平滑肌细胞的影响 |
| Expression of MAD1 gene in aortic dissection and its effect on aortic smooth muscle cells |
| 投稿时间：2019-02-01 修订日期：2019-04-17 |
| DOI：10.15972/j.cnki.43-1509/r.2019.03.005 |
| 中文关键词: 主动脉夹层 主动脉平滑肌细胞 MAD1基因 细胞凋亡 |
| 英文关键词:aortic dissection aortic smooth muscle cells MAD1 gene apoptosis |
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| 中文摘要: |
| 收集病变的升主动脉中膜组织标本为主动脉夹层组；收集同期在本院行尸检且无手术史、大血管病变史的升主动脉壁内组织标本18份为对照组；RT-PCR和Western blot检测有丝分裂阻滞缺陷蛋白(MAD1) mRNA和蛋白表达水平。在体外建立MAD1基因高表达人主动脉血管平滑肌细胞(HA-VSMC)模型,检测细胞增殖和凋亡情况以及凋亡相关蛋白。结果显示主动脉夹层组患者主动脉夹层中MAD1 mRNA和蛋白相对表达量明显高于对照组(P<0.05)。随着转染时间的延长,各组细胞增殖水平均逐渐增加,转染48 h、72 h后MAD1过表达组细胞增殖情况低于空白对照组及空载体转染组(P<0.01)。转染48 h后,MAD1过表达组细胞凋亡率明显高于空白对照组及空载体转染组(P<0.01)。MAD1过表达组含半胱氨酸的天冬氨酸蛋白水解酶Caspase-3、Caspase-8、Caspase-9 mRNA和蛋白相对表达量明显高于空白对照组及空载体转染组(P<0.05)。实验结果表明,MAD1过表达可通过抑制细胞增殖,激活细胞外凋亡途径和细胞内凋亡途径来促进人主动脉平滑肌细胞HA-VSMC细胞凋亡,参与主动脉夹层的发生与发展。 |
| 英文摘要: |
| patients with aortic dissection who underwent surgical treatment in our hospital were selected as the study group, and 18 ascending aortic tissue specimens without history of operation and macrovascular disease were collected as the control group. The expression of MAD1 was detected by RT-PCR and Western blot. The model of human aortic vascular smooth muscle cells (HA-VSMC) with high MAD1 gene expression was established in vitro by adenovirus vector transfection technology. The cell proliferation and apoptosis were detected.The results showed that The relative expression of MAD1 in aortic dissection of the study group was significantly higher than that of the control group (P<0.05). With the prolongation of transfection time, the cell proliferation level of each group increased gradually. The cell proliferation of MAD1 overexpression group was lower than that of blank control group and blank vector transfection group 48 hours and 72 hours after transfection, and the difference was statistically significant (P<0.01). After 48 hours of transfection, the apoptotic rate of MAD1 overexpression group was significantly higher than that of blank control group and blank vector transfection group (P<0.01). The relative expression of Caspase-3, Caspase-8, Caspase-9 in MAD1 overexpression group was significantly higher than that in blank control group and blank vector transfection group (P<0.05). So overexpression of MAD1 can promote apoptosis of HA-VSMC cells by inhibiting cell proliferation, activating extracellular and intracellular apoptotic pathways, and participate in the occurrence and development of aortic dissection. |
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