| 谢婷,廖湘平,黄丽艳,欧志兵,张健,罗迪贤,刘文培.地塞米松体外下调Eomes表达抑制1型辅助性T细胞功能.[J].中南医学科学杂志.,2019,(2):130-134. |
| 地塞米松体外下调Eomes表达抑制1型辅助性T细胞功能 |
| Dexamethasone inhibit T helper 1 cell function via down-regulating eomes expression in vitro |
| 投稿时间：2018-11-21 修订日期：2019-02-24 |
| DOI：10.15972/j.cnki.43-1509/r.2019.02.004 |
| 中文关键词: 地塞米松 辅助性T细胞 转录 功能 |
| 英文关键词:dexamethasone T helper cells transcription function |
| 基金项目: |
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| 中文摘要: |
| 本文使用地塞米松处理健康人外周血单个核细胞(PBMCs)72 h后,探究其对CD4+T细胞及其亚群的频数、增殖、凋亡比例；功能性细胞因子及关键转录因子表达的影响。结果显示,地塞米松组CD4+T、2型辅助性T细胞(Th2)、Th17细胞比例增加(P<0.01,P<0.05,P<0.01),Th1细胞比例降低(P<0.01)。Th1细胞上γ-干扰素(IFN-γ)、颗粒酶B(GzmB)、脱中胚蛋白(Eomes)比例、Eomes与T盒转录因子(T-bet)比值均降低(均P<0.01),晚期凋亡细胞比例增加(P<0.05)。提示地塞米松体外处理PBMCs促进Th1细胞凋亡,改变辅助性T细胞亚群的分布,并通过抑制Th1细胞转录因子Eomes的表达进而抑制其功能。 |
| 英文摘要: |
| To investigate the expression of CD4+T cell subsets, proliferation, apoptosis and transcription factors, measured by flow cytometry after dexamethasone treatment for 72 hours. Intracellular cytokines IFN-γ and Granzyme B were measured after stimulation with PMA/Ionmycin. Compared to the control group, the frequency of CD4+T cells, Th2 and Th17 cells were significantly increased (P<0.01, P<0.05, P<0.01), while Th1 frequency was decreased significantly (P<0.01). The expression of IFN-γ, Granzyme B, transcription factor Eomesodermin and the ratio of Eomesodermin/T-bet in Th1 cells were decreased significantly (P<0.01, P<0.01, P<0.01, P<0.01). The proliferation and early apoptosis of Th1 cells had no difference, while the frequency of late apoptosis in Th1 cells was increased (P<0.05). Our studies showed that treatment with dexamethasone for 72 hours in vitro, advanced Th1 cells apoptosis, decreased the frequency of Th1 cells, altered the distribution of helper T cells, inhibiting the function of Th1 cells via reducing the expression of transcription factor Eomes. |
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