| 李健,周峥,熊立新,邓科平,尹清华,何恒正.RIP1在肝癌组织中的表达意义及其对细胞侵袭、迁移的影响.[J].中南医学科学杂志.,2019,(1):20-24. |
| RIP1在肝癌组织中的表达意义及其对细胞侵袭、迁移的影响 |
| The clinicopathological study of receptor-interacting protein kinase-1(rip1) on liver cancer and its effect on cell invasion and migration |
| 投稿时间：2018-04-03 修订日期：2018-12-08 |
| DOI：10.15972/j.cnki.43-1509/r.2019.01.005 |
| 中文关键词: 肝癌 受体相互作用蛋白激酶-1 组织病理 细胞侵袭 细胞迁移 |
| 英文关键词:Liver cancer Receptor-interacting protein kinase-1 Histopathology Cell invasion Cell migration |
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| 中文摘要: |
| 探讨受体相互作用蛋白激酶-1(RIP1)在肝癌组织及癌旁组织中的表达情况,并探索其对肝癌细胞侵袭和迁移的分子调控机制。收集肝癌和癌旁组织,免疫组化检测RIP1蛋白的表达,筛选高表达RIP1的肝癌细胞系。对肝癌细胞系进行RIP1沉默处理并设置阴性对照。采用western blot及RT-PCR法检测RIP1的表达量；Tranwell观察细胞侵袭能力；细胞划痕观察细胞迁移能力。结果显示, 48例肝癌组织中,22例存在RIP1蛋白阳性表达,阳性率为45.83%,其中,强阳性患者为15例,中等及弱阳性7例,该研究中有6例复发患者。RIP1在肝癌组织中的表达明显高于癌旁组织(P＜0.05),高表达RIP1患者的总生存率和无复发生存率显著低于低表达RIP1的患者(均P＜0.05)。抑制RIP1基因后其相对表达量、细胞侵袭、迁移能力均下降(P＜0.05)。RIP1在肝癌中存在高表达,可能参与肝癌细胞侵袭与迁移过程。 |
| 英文摘要: |
| This study is aimed to investigate the expression of receptor-interacting protein kinase-1(RIP1) in hepatocellular carcinoma and its relationship with histopathology, and to explore its effect on the invasion and migration of hepatoma cells. Tumor tissues and paracancerous tissues were collected; the expression of RIP1 in the hepatocellular carcinoma cell line was screened and the cell line with the highest expression of RIP1 was collected for subsequencing experiments.The RIP1 gene was inhibited and normal control group was set. Western blotting and RT-PCR were used to detect the expression of RIP1. Tranwell was used to observe the invasive ability of the cells. Cell migration was observed in cell scratches. Of the 48 patients with liver cancer, 22 were stained, and the positive rate of staining was 45.83%. Of these, 15 were strongly positive, 7 were moderately and weakly positive, and 6 were relapsed. The expression of RIP1 in hepatocellular carcinoma was significantly higher than that in paracancerous tissue(P＜0.05). Total survival rate and relapse-free survival rate in patients with high expression of RIP1 was significantly higher than that in patients with low expression of RIP1(P＜0.05). After inhibiting RIP1 gene, its relative expression, cell invasion and migration ability were all decreased(P＜0.05). RIP1 is high expressed in liver cancer and it may participate in the invasion and migration of liver cancer cells. |
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